Hieff NGS^TM OnePot Pro DNA Library Prep Kit for Illumina^TM is a next-generation enzymatic DNA fragmentation-based library preparation kit specifically designed for the Illumina^TM high-throughput sequencing platform. Compared to traditional library prep methods, this kit employs a high-quality fragmentation enzyme, eliminating the need for laborious sonication. It simplifies the workflow by combining the fragmentation and end-repair modules into a single step, significantly reducing both time and cost. The kit delivers excellent library conversion efficiency and is suitable for various sample types, including standard plant and animal genomes, microbial genomes, and FFPE DNA. The improved version of the kit features an optimized ligase to reduce adapter self-ligation, and a novel high-fidelity polymerase to further enhance amplification uniformity and fidelity.

Features

Broad input range: 500 pg – 1 µg DNA or cDNA, compatible with challenging FFPE DNA samples

Fast one-tube workflow: fragmentation, end repair & dA-tailing in a single step

High library conversion rate: High-fidelity enzyme for superior yield and library quality

Low sequence bias, reliable results across applications

Consistent performance with strict quality control

Components

Storage

This product should be stored at -25~-15℃ for 1 year.

Application

DNA library Preparation; Whole genome sequencing(WGS); whole exome or other targeted capture sequencing; amplicon sequencing; immunoprecipitation sequencing; metagenomic sequencing.

Figures

1. Workflow

2. Flexible and High-Efficiency Fragmentation

Figure 1. Efficient fragmentation and library preparation of 200 ng Human gDNA using the OnePot Pro DNA Library Prep Kit. Fragmentation efficiency was evaluated at different incubation times, and high-quality libraries were obtained with a 20-min fragmentation and 5 amplification cycles.

3. Low GC Bias

Figure 2. GC bias analysis of ZYMO standard D6306 (10 ng input). Libraries were prepared using Yeasen #12205 and Supplier K* kits. Sequencing results from three species with different GC contents showed comparable GC bias profiles between the two kits.

4. Superior performance

Figure 3. Comparison of coverage uniformity and self-ligation artifacts in hybrid capture sequencing of tumor gDNA standard. Starting with 50 ng GW-OGTM006 DNA, libraries were prepared using Yeasen #12205, supplier V*, and supplier K* kits following their manuals. Libraries were size-selected with 0.7×/0.2× dual-bead purification, and 500 ng of each was captured using the IDT xGen Pan-Cancer Panel (801,904 bp). Sequencing was performed on the Illumina PE150 platform with equal data volumes analyzed. Results demonstrated that Yeasen #12205 achieved superior coverage uniformity and reduced self-ligation artifacts compared with other suppliers.

Documents

Safety Data Sheet

12205_MSDS_HB250923_EN.PDF

Manuals

12205_Manual_Ver.EN20250923.pdf