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Common Problems and Solutions in Annexin V-Based Flow Cytometry Apoptosis Assays

Common Problems and Solutions in Annexin V-Based Flow Cytometry Apoptosis Assays

Zhang A, Pan S, Zhang Y, et al. Carbon-gold hybrid nanoprobes for real-time imaging, photothermal/photodynamic and nanozyme oxidative therapy. The...
Common Problems and Solutions in Annexin V-Based Flow Cytometry Apoptosis Assays

Common Problems and Solutions in Annexin V-Based Flow Cytometry Apoptosis Assays

Zhang A, Pan S, Zhang Y, et al. Carbon-gold hybrid nanoprobes for real-time imaging, photothermal/photodynamic and nanozyme oxidative therapy. The...
What to Do When Mitochondrial Labeling Goes Wrong? Common Abnormalities and Solutions

What to Do When Mitochondrial Labeling Goes Wrong? Common Abnormalities and Solutions

Mitochondria are involved in various cellular processes, including autophagy, calcium homeostasis, immune response, signal transduction, and apopto...
How to Label Exosomes? High-Efficiency Exosome Fluorescent Labeling FAQ

How to Label Exosomes? High-Efficiency Exosome Fluorescent Labeling FAQ

Exosomes are membrane-bound vesicles actively secreted by cells, typically 30–150 nm in diameter. They contain various biomolecules derived from ho...
Cell Viability and Proliferation: Unlocking the Code of Cellular "Vitality"

Cell Viability and Proliferation: Unlocking the Code of Cellular "Vitality"

As the basic unit of life, the vitality of cells directly reflects an organism’s health status and disease progression. Assessing cell viability is...
Revisiting Technical Challenges in Tumor Cell Invasion and Migration Assays: Causes and Optimization Strategies

Revisiting Technical Challenges in Tumor Cell Invasion and Migration Assays: Causes and Optimization Strategies

Inhibited Migration to the Lower Chamber This issue may result from an insufficient chemotactic gradient or the absence of effective chemoattracta...
Common Issues in Liposome Transfection

Common Issues in Liposome Transfection

Transfection is one of the most widely used techniques in cell-based experiments. It involves introducing exogenous nucleic acids into cells using ...
FAQs on Mycoplasma Elimination

FAQs on Mycoplasma Elimination

Figure 1. Electron Micrograph of Mycoplasma Adhering to Host Cells Background and Hazards of Mycoplasma Contamination Mycoplasmas are the smalle...
Cell Contamination Solutions

Cell Contamination Solutions

Sources of Cell Contamination Physical Contamination: Radiation, temperature fluctuations, etc. Chemical Contamination: Impurities in media, seru...
Transfection Reagent Specifically for AAV Packaging: Halved Reagent Usage with Unchanged Viral Yield (E12 VG/mL)

Transfection Reagent Specifically for AAV Packaging: Halved Reagent Usage with Unchanged Viral Yield (E12 VG/mL)

In the rapidly evolving field of Cell and Gene Therapy (CGT), producing Adeno-Associated Virus (AAV) vectors efficiently remains a significant indu...
Mycoplasma Tests and Contamination Total Solutions

Mycoplasma Tests and Contamination Total Solutions

Mycoplasma Detection, Prevention, and Removal - All in One Step! Mycoplasma Mycoplasma, also known as mycoplasm, is the smallest and simplest pro...
Hieff Trans ultraAAV Transfection Reagent GMP Edition: 50% Less Reagent, Same High Yield

Hieff Trans ultraAAV Transfection Reagent GMP Edition: 50% Less Reagent, Same High Yield

Efficient production of AAV (adeno-associated virus) vectors is a critical challenge in cell and gene therapy (CGT). Traditional transfection reage...
Culture method of mouse bone marrow-derived dendritic cells (BMDC) and selection of cytokines

Culture method of mouse bone marrow-derived dendritic cells (BMDC) and selection of cytokines

1. What are dendritic cells (DC)? Dendritic cells (DCs) are the most effective antigen presenting cells (APCs) in the human body. DCs are the onl...
Application of Ceturegelâ„¢ Matrix Gel in Angiogenesis Experiments

Application of Ceturegelâ„¢ Matrix Gel in Angiogenesis Experiments

Angiogenesis is closely associated with wound healing, inflammation, rheumatoid arthritis, diabetic retinopathy, macular degeneration, and tumor gr...

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