Pulmonary macrophages are a class of immune cells located in the lung tissue and are part of the mononuclear phagocyte system. Lung macrophages are derived from two main pathways: first, monocytes produced in the bone marrow enter the lungs and differentiate into macrophages; and second, resident macrophages are inherent within the lungs. The main types are Alveolar Macrophages, AMs, and Interstitial Macrophages, IMs.
Alveolar macrophages have the following functions：
- Immunosurveillance: lung macrophages continuously monitor lung tissues for potential threats such as inhaled pathogens, dust particles, and cellular debris.
- Phagocytosis: removal of pathogens, foreign bodies and apoptotic cells by phagocytosis to maintain clean lungs.
- Inflammatory response: in the presence of infection or injury, lung macrophages rapidly activate, release inflammatory mediators (e.g., cytokines and chemokines), and recruit other immune cells to participate in the inflammatory response.
- Tissue repair: after inflammation subsides, lung macrophages participate in tissue repair and regeneration, promoting recovery of damaged tissues.
- Antigen presentation: lung macrophages can take up and process antigens and present them to T cells to initiate adaptive immune responses.
- Metabolic regulation: involved in metabolic functions in the lungs, such as lipid metabolism and iron metabolism.
Previously, we introduced the application of Clodronate Liposomes in the liver, and today we share the article and methodology related to the use of Clodronate Liposomes to remove macrophages from mouse lungs.
Literature 1
Article Source: Murine alveolar macrophages limit replication of vaccinia virus.
Macrophage Clearance Methods:
Tracheal Injection 100 uL Clodronate Liposomes.
Results are shared:
Total numbers of cells recovered from bronchoalveolar lavage fluid were quantified at different time points after treatment (n = 3 per time point). Data are expressed as the percent of cells recovered relative to mice that did not receive either clodronate or saline liposomes (n = 3).
Literature 2
Article Source: Alveolar macrophages are sentinels of murine pulmonary homeostasis following inhaled antigen challenge
Macrophage Clearance Methods:
Once weekly, 50 μL of Clodronate Liposomes were administered intratracheally.Lung macrophage counts were quantified by flow cytometry at 1, 4, and 7 days after administration.
Results are shared:
Literature 3
Article Source: Respiratory macrophages regulate CD4 T memory responses to mucosal immunization with recombinant adenovirus-based vaccines
Macrophage Clearance Methods:
Clodronate Liposomes (2 mg/20 g) depleted macrophages by intraperitoneal injection. Flow cytometry analysis of CD11c and F4/80 expression in spleen and lung was performed.
Results are shared:
Literature 4
Article Source: CD11c+/CD11b+ Cells Are Critical for Organic Dust–Elicited Murine Lung Inflammation
Macrophage Clearance Methods:
Intranasal 30 μL Clodronate Liposomes were administered every 3 - 4 days for 3 weeks to maintain macrophage depletion.
Results are shared:
Since the mouse models in the above studies vary, the doses and methods mentioned in the literature are for reference only. Appropriate adjustments and optimization for your specific experimental conditions are recommended.
In addition, there are different dosing methods for macrophages residing in different tissues. We will continue to collate relevant literature to provide you with more references. If you have any specific tissue site or macrophage removal method needs, please let us know in the message area and we will prioritize the collation of relevant content.
Product Recommendation
|
Product name |
Item number |
Specification |
|
40337ES08 |
5 mL |
|
|
40337ES10 |
10 mL |
|
|
40338ES08 |
5 mL |
|
|
40338ES10 |
10 mL |