Alternative Names

CEACAM-5, CD66e, CEA, Meconium antigen 100, Carcinoembryonic, CEACD66e, DKFZp781M2392

Species

Human

Expression Sequence

Lys35-Ala685

Accession

P06731-1

Tag

C-His-Avi

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 74.23 kDa. Due to glycosylation, the protein migrates to 110-150 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Biotinylated Human CEACAM-5, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CEACAM Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.