وصف
Interleukin-3 (IL-3) is a hematopoietic growth factor that promotes the survival, differentiation, and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil, and mast cell lineages. IL-3 is produced by T cells, mast cells, and eosinophils, and can enhance thrombopoiesis, phagocytosis, and antibody-mediated cytotoxicity. Its ability to activate mononuclear cells suggests that IL-3 may have additional immunomodulatory roles. Many IL-3 activities depend on co-stimulation with other cytokines.
Recombinant IL-3 is a 15.0 kDa recombinant protein containing 133 amino acid residues. This product is expressed in E. coli and provided as a lyophilized powder. It features high activity, high purity, low endotoxin, and is tag-free.
Specifications
|
Cat.No. |
90212ES10 / 90212ES50 / 90212ES60 / 90212ES80 |
|
Size |
10 μg / 50 μg / 100μg / 1mg (10×100μg) |
Components
|
Name |
90212ES10 |
90212ES50 |
90212ES60 |
90212ES80 |
|
Recombinant Human IL-3 GMP Protein |
10 μg |
50 μg |
100μg |
1mg (10×100μg) |
Performance parameters
|
Synonyms |
Interleukin-3; IL-3; Hematopoietic growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor |
|
Source |
E. coli |
|
Sequence |
Ala20-Phe152. |
|
Molecular Weight |
Approximately 15.08 kDa. |
|
Accession |
P08700 |
|
Tag |
No Tag |
|
Biological Activity |
The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7×106 IU/mg |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
< 0.01 EU/μg protein as determined by LAL method. |
|
Sterility |
Negative. |
|
Mycoplasma |
Negative. |
|
In Vitro Virus |
Negative. |
|
Host Cell Protein |
Less than 0.5 ng/ug when tested by ELISA. |
|
Host Cell DNA |
Less than 20 ng/mg when tested by qPCR. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0. |
|
Physical Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
Storage
Store at -25~-15°C, valid for 3 years.After reconstitution, store at 2~8°C, valid for 30 days. After reconstitution, store at -85~-65°C, valid for 3 months.
Reconstitution Method
It is recommended to reconstitute Human IL-3 in sterile PBS to a concentration of 0.1-1.0 mg/mL. Avoid repeated freeze-thaw cycles as much as possible.
Product Data
SDS- PAGE
Human IL-3 on SDS-PAGE under reduced condition. The purity is greater than 95%.
Cell Based Assay
The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7 × 106 IU/mg.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves,for your safety.
Documents
Safety Data Sheet
Manuals:
Payment & Security
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
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