Alternative Names

GDF-15, MIC-1, NAG-1, PDF, PLAB, PTGFB, GDF15, MIC1, RG-1, Placental TGF-beta, PTGF-beta, PTGFBPTGF-beta, Placental TGF-β, PTGF-β, PTGFBPTGF-β

Species

Canine

Expression Sequence

Ala197-Val307

Accession

A0A8C0TNP6

Tag

N-His

Expression System

E.coli

Molecular Weight

The protein has a predicted MW of 13.12 kDa. The protein migrates to 15-20 kDa under reduced (R) condition and 23-26 kDa under Non reducing (N) condition based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Canine GDF15, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA . 【ELISA Data】 Immobilized Canine GDF15, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 48.3ng/ml determined by ELISA.

Reconstitution Method

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).