Alternative Names

TGF beta1,TGFB1,DPD1,CEDLAP

Species

Human

Expression Sequence

Leu30-Ser390(C33S)

Accession

P01137

Tag

N-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa(LAP) / 13-15 kDa(Mature TGF Beta 1) kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Human Latent TGF beta 1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 86.1ng/ml determined by ELISA. 【ELISA Data】 Immobilized Human Latent TGF beta 1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB8, His Tag with the EC50 of 6.6ug/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.