The Live Cell Analysis Kit contains SuperView™ 488 Caspase-3 Substrate and Ac-DEVD-CHO Caspase-3/7 Inhibitor. The SuperView™ 488 Caspase-3 Substrate provides an effective tool for detecting cell apoptosis based on Caspase-3/7 activity, suitable for fluorescence microscopy observation and flow cytometry analysis.

Compared to other fluorescent substrates or inhibitors for Caspase analysis based on FLICA (Fluorochrome-Labeled Inhibitors of Caspases), the Substrate provided in this kit detects Caspase-3/7 activity without inhibiting the apoptosis process in intact cells. The Substrate consists of a fluorescent DNA dye coupled with the Caspase-3/7 DEVD recognition sequence. Initially non-fluorescent, the Substrate crosses the cell membrane into the cytoplasm. In apoptotic cells, Caspase-3/7 cleaves the Substrate, releasing a high-affinity DNA dye. This dye migrates to the nucleus, labels the DNA, and emits bright green fluorescence. The Substrate is bifunctional: it detects Caspase-3/7 activity while simultaneously visualizing morphological changes in the nucleus during apoptosis. SuperView™ staining is compatible with formaldehyde fixation and subsequent immunostaining experiments.

Spectral Properties of SuperView™: Ex/Em = 500/530 nm (when bound to DNA)

Features

• Dual-Function Detection: Simultaneously visualizes Caspase-3/7 activity and apoptotic nuclear morphology in live cells.
• Downstream Compatibility: Fully compatible with formaldehyde fixation and subsequent immunostaining protocols.
• Non-Inhibitory: Detects apoptosis without interfering with the cell death process.

Components

Storage

This product should be stored at 2~8℃ for 1 year.

Figure

 Figure 1. Comparison of Flow Cytometry Scatter Plots (SSC-A vs. BL1-A) for Two Batches.

Left (Lot 1) and right (Lot 2) panels show SSC-A vs. BL1-A fluorescence. The vertical line gates low (left) and high (right) BL1-A expressing cells. Lot 1: 81.9% low / 18.1% high. Lot 2: 85.7% low / 14.3% high. Both batches show similar distribution patterns.

Documents:

Safety Data Sheet

40273_MSDS_HB260324_EN.PDF

Manuals

40273_Manual_Ver.EN20260324.pdf

FAQ

Q: Can it be used for staining tissue sections?

A: No, it is designed exclusively for live cell staining.

Q: Is it mandatory to use Hoechst 33342 live cell staining solution when observing with a fluorescence microscope?

A: No, it is primarily used to facilitate the observation of the positive rate. You may choose to use it based on your specific experimental needs.

Q: Do the measured values represent activity or concentration?

A: They represent activity.

Reference

[1] Du W, Wang L, Liao Z, Wang J. Circ_0085289 Alleviates the Progression of Periodontitis by Regulating let-7f-5p/SOCS6 Pathway. Inflammation. 2021;44(4):1607-1619. doi:10.1007/s10753-021-01445-8(IF:4.092)

[2] Zhu L, Shi D, Cao J, Song L. LncRNA CASC2 Alleviates Sepsis-induced Acute Lung Injury by Regulating the miR-152-3p/PDK4 Axis. Immunol Invest. 2022;51(5):1257-1271. doi:10.1080/08820139.2021.1928693(IF:3.657)

[3] Teng Z, Sun X, Guo Y, Zhang M, Liu Y, Xu M. Curcumae longae Rhizoma (Jianghuang) extract reverses the 5-Fluoruracil resistance in colorectal cancer cells via TLR4/PI3K/Akt/mTOR pathway [published online ahead of print, 2022 Jun 13]. Clin Res Hepatol Gastroenterol. 2022;101976. doi:10.1016/j.clinre.2022.101976(IF:3.189)