These precast gels utilize a Bis-Tris buffer system and feature plastic cassettes, offering superior separation performance with sharper, clearer bands. Compared to conventional Tris-Glycine systems, they provide stronger buffering capacity. The neutral running buffer enhances gel stability and prevents protein re-modification during electrophoresis. A proprietary casting technology ensures consistent batch-to-batch quality and uniform band distribution.

Components

[Note]: The recommended running buffer is Precast Running Buffer (Tris-MOPS), 2 L (Powder) (Cat#36271ES).

Storage

This product should be stored at 2~8℃ for one year. Do not freeze.

Instructions

1. Remove the Precast Protein Plus Gel from its packaging, peel off the gold tape from the bottom of the cassette, gently remove the comb, and secure the gel cassette in the electrophoresis tank.

2. Fill the inner chamber with Tris-MOPS-SDS Running Buffer. Add buffer to the outer chamber until the liquid level reaches at least 1/3 of the gel cassette height. Use a pipette to gently flush the wells to remove any residual storage buffer or impurities.

3. Load samples by vertically inserting the pipette tip into the well. Avoid puncturing the gel or inserting the tip too deeply, as this may deform the cassette and cause sample leakage.

4. Electrophoresis Conditions: Run at 150 V for 50–70 minutes. Stop electrophoresis when the bromophenol blue dye front reaches the bottom of the gel or the desired position. For sharper, straighter bands, reduce the voltage to 100–120 V and extend the running time accordingly.

5. After electrophoresis, remove the gel cassette. Insert a gel opener or similar tool into the gap between the two plates. Gently pry open the cassette at the top, middle, and bottom positions, then repeat on the opposite side until the cassette is fully separated.

6. The gel may adhere to either plate. Tilt the plate with the gel over a container of water and gently manipulate the gel to allow it to drop freely into the water. Rinse the gel by gently agitating the container, then proceed with staining or western blotting.

Notes

1. The gold tape on the bottom of the cassette must be removed; otherwise, protein separation will not occur.

2. Ensure the electrophoresis system is compatible. Incompatibility may cause buffer leakage between the inner and outer chambers, which can halt electrophoresis.

3. If buffer leakage occurs due to the thinness of the cassette, use a special dam to increase the thickness. YeaSen provides a compatible dam (Cat#80837ES).

4. For Bio-Rad Mini-PROTEAN systems, remove the U-shaped gasket with protrusions and reinstall it with the smooth side facing outward to prevent leakage (see figure).

5. The plastic plates feature an anti-adhesion coating. If the gel sticks, rinse it off with ultrapure water.

6. If the gel becomes distorted or protrudes after removal, trim the lower edge of the gel along the upper edge of the four cutout sections using a gel scraper.

7.The precast gel cassettes are tightly sealed. Use a metal tool or similar object to pry them open. YeaSen provides a specialized Gel Opener (Cat#80838ES).

8. This product is for research use only.

9. Please operate with lab coats and disposable gloves，for your safety.

Recommended Related Products

Protein migration chart for the Precast Protein Plus Gel Bis-Tris system.

Reference Formula for Electrophoresis Running Buffer

Concentrations of components for denaturing protein electrophoresis buffer: 50 mM Tris, 50 mM MOPS, 0.1% SDS, 1 mM EDTA.

Documents:

Safety Data Sheet

36264_MSDS_HB260701

Manuals:

36264_Manual_HB20260701