The Hifair™ CoAmp Multiplex One Step RT-qPCR Probe Kit (UDG Plus) is a multiplex one-step fluorescent quantitative PCR master mix designed for the efficient simultaneous detection of DNA and RNA targets within the same reaction system. By carefully integrating high-performance thermostable reverse transcriptase and hot-start DNA polymerase, along with a proprietary reaction buffer system, this kit achieves excellent amplification efficiency and balanced detection performance for both DNA and RNA templates. It effectively overcomes the amplification bias issues commonly encountered with traditional reagents during DNA/RNA co-detection.

The core components of the kit include the deeply optimized 5× Hifair™ MP Buffer and a master mix enzyme system. The buffer contains sufficient Mg²⁺, dNTPs, and other essential components for amplification, and incorporates specific amplification enhancers and multiplex reaction stabilizers. This ensures high amplification efficiency while supporting robust multiplex detection of up to four targets. Additionally, the system incorporates dUTP and Uracil-DNA Glycosylase (UDG), which efficiently degrades carryover PCR products, fundamentally preventing amplification product contamination and ensuring the reliability and reproducibility of experimental results.

Features

Dual-Detection, Doubled Throughput: Simultaneous DNA & RNA amplification in a single tube saves 50% of reaction wells and doubles testing capacity.

Rapid Results: Delivers both DNA and RNA pathogen results in a single reaction, accelerating clinical and veterinary decision-making.

Versatile & Cost-Effective: Compatible with both DNA and RNA assays, streamlining production and reducing costs for large-scale manufacturing.

High Performance & Stability: Achieves a sensitivity of 250 copies/mL, remains stable after 14 days at 37°C, supports rapid cycling, and is compatible with all major real-time PCR instruments.

Components

[Note]: 1. 1.5× Hifair™ MP Buffer is an abbreviation for Hifair™ Multiplex One Step RT-qPCR Probe Kit (UDG Plus).

2. Hifair™ Enzyme Mix mainly contains thermostable Reverse Transcriptase and HotStart Taq DNA Polymerase, RNase inhibitor, UDGase, etc.

Storage

This product should be stored at -25~-15℃ for 1 year.

Application

Co-detection of DNA & RNA Pathogen. Pathogen Multiplex Assay

Figures

1. Efficient Single-Tube DNA/RNA Co-amplification  

Figure 1. Co-detection of DNA and RNA pathogens.

Using Reagent 16929, the simultaneous detection of African Swine Fever Virus (DNA) and Porcine Reproductive and Respiratory Syndrome Virus (RNA) was performed in a single reaction well. Results demonstrate that both targets achieved amplification efficiencies exceeding 90%, with no amplification bias, ensuring balanced and reliable outcomes.

2. Strong Stability

Figure 2. Stability assessment

(A) Accelerated Stability: After incubation at 37°C for 14 days, Reagent 16929 showed no changes in detection rate, Ct values, or peak shape compared to the -20°C control, demonstrating excellent thermal stability.

(B) Freeze-Thaw Stability: The reagent maintained consistent amplification performance for both DNA-HPIV and RNA-HCOV after 50 dry ice freeze-thaw cycles.

Documents:

Safety Data Sheet

16929_MSDS_HB260609

Manuals

16929_Manual_HB20260609