T3 DNA Ligase is derived from bacteriophage T3 and is an ATP-dependent double-stranded DNA ligase. It efficiently catalyzes the formation of a phosphodiester bond between adjacent 5′-phosphate and 3′-hydroxyl groups in double-stranded DNA, with higher ligation efficiency for A/T-overhang termini compared to C/G-overhangs.

T3 DNA Ligase effectively ligates both cohesive (sticky) ends and blunt ends (blunt-end ligation efficiency can be enhanced by addition of PEG 6000). It also repairs single-strand nicks in double-stranded DNA or DNA–RNA hybrid duplexes. During nick sealing, the intact strand must be DNA, while the nicked strand may have any combination at its 5′ and 3′ termini—DNA–DNA, DNA–RNA, RNA–DNA, or RNA–RNA—all of which are substrates for T3 DNA Ligase. Consequently, this enzyme can be used to generate DNA–RNA or RNA–DNA chimeric linkages and to perform DNA template-directed RNA ligation for the synthesis of longer RNA fragments.

Additionally, T3 DNA Ligase exhibits higher tolerance to NaCl than T4 DNA Ligase.

Features

High Purity: Protein purity ≥99% (by SDS-PAGE)

Low Contaminants: Free of detectable exonuclease, nicking endonuclease, non-specific nuclease, and RNase activity

High Activity: Performance comparable to leading imported brands

Specifications

Components

Storage

This product should be stored at -25~-15℃ for 2 years.

Application

Molecular cloning

Ligation of adapters or linkers to double-stranded DNA

Long-chain synthesis of antisense oligonucleotide (ASO) therapeutics

Circularization of linear DNA

Repair of nicked sites in double-stranded DNA

Formation of DNA–RNA chimeric junctions

DNA template–directed RNA ligation for the assembly of long RNA fragments

Figures

High Activity

Figure 1. Comparison of ligation efficiency between Yeasen T3 DNA Ligase and imported Supplier A.

Yeasen T3 DNA Ligase and Supplier A’s ligase were tested at different input amounts using 5 pmol sticky-end substrates. The results show that Yeasen T3 DNA Ligase achieves high-efficiency ligation comparable to the leading brand’s product.

Documents:

Safety Data Sheet

14965_MSDS_HB251211_EN.PDF

Manuals

14965_Manual_Ver.EN20251211.pdf