T4 RNA Ligase 2, truncated KQ is a double-point mutant (R55K, K227Q) of the truncated form of T4 RNA Ligase 2. While maintaining high ligation efficiency, it significantly reduces non-specific RNA ligation (e.g., RNA concatemerization or circularization). This enzyme specifically ligates a 5′-pre-adenylated DNA or RNA adapter to the 3′-OH end of an RNA molecule, making it highly suitable for adapter ligation to small RNAs in cDNA library construction.ATP is not required for this reaction, but the adapter must be pre-adenylated at the 5′ end.

Features

• Protein purity ≥ 95%
• Free of detectable nuclease, nicking enzyme, RNase, and phosphatase contamination
• High ligation activity

Application

Small RNA Library preparation

Specifications

Components

Storage

This product should be stored at -25~-15℃ for 2 years. 

Figures

1. High ligation activity

Figure 1. Ligation Performance of T4 RNA Ligase 2, Truncated KQ

T4 RNA Ligase 2, Truncated KQ from Yeasen and Supplier A* was tested at varying enzyme inputs in ligation reactions containing a 31-nt single-stranded RNA (ssRNA) substrate (Substrate 1) and a 17-nt pre-adenylated ssRNA substrate (Substrate 2). Results demonstrate that Yeasen’s enzyme efficiently ligates ssRNA substrates, delivering performance comparable to that of Supplier A*.

2. No residual exonuclease, nicking endonuclease, or RNase detected

Figure 2. Nuclease Contamination Testing of T4 RNA Ligase 2, Truncated KQ

200 units of T4 RNA Ligase 2, Truncated KQ from three independent batches (Yeasen) were incubated with nucleic acid substrates, and reaction products were analyzed by agarose gel electrophoresis. No detectable exonuclease, nicking endonuclease, or RNase activity was observed in any batch, ensuring high reliability and accuracy for sensitive downstream applications.

Documents:

Safety Data Sheet

14653_MSDS_HB251218_EN.PDF

Manuals

14653_Manual_Ver.EN20251218.pdf