Cell Transfection Solutions

Validated, high-efficiency transfection protocols for a wide range of cell types. Our solutions help you achieve optimal results with minimal optimization—often right from the first attempt. We provide cell-specific protocols for both primary cells and common cell lines, covering cell growth, culture conditions, passaging, and key steps for efficient transfection. These optimized protocols include detailed step-by-step instructions to achieve high transfection efficiency with low cytotoxicity. Verified by numerous academic and research institutions worldwide, our solutions are designed to meet real-world experimental needs and help you reach your research goals faster.

Transfection Reagent Selection Guide

Cat.NO.	40801ES	40802ES	40803ES	40804ES	40806ES
Product Name	Booster Transfection Reagent	Liposomal Transfection Reagent	siRNA/miRNA Transfection Reagent	mRNA Transfection Reagent	Insect Cell Transfection Reagent
Cell Type	Common cells	Common cells	Common cells	Common cells	Sf9, High Five
	Hard-to-transfect cell			Hard-to-transfect cell	Hard-to-transfect cell
	Primary cells				Primary cells
	Stem cells				Stem cells
Nucleic Acid Type	DNA	DNA	DNA	Lentivirus
	siRNA				siRNA
	miRNA				miRNA
	ASO				ASO
	mRNA
Application	For transfection of various hard-to-transfect cell lines, including nucleated and primary cells	For DNA transfection in multiple cell types	For viral packaging and protein expression	Primarily used to enhance lentiviral infection	For siRNA and miRNA transfection, enabling high expression in various hard-to-transfect cells

Cat.NO.	40808ES	40809ES	40810ES	40815ES	40816ES
Product Name	293 Cell Transfection Reagent	PEI Virus Packaging Reagent	PEI-GMP Virus Packaging Reagent	UltraAAV Virus Packaging Reagent	UltraAAV-GMP Virus Packaging Reagent
Cell Type	Common cells	Common cells	Common cells	Common cells	Common cells
	Hard-to-transfect cell	Hard-to-transfect cell
	Primary cells	Primary cells
	Stem cells	Stem cells
Nucleic Acid Type	DNA		DNA	DNA	DNA
	siRNA
	miRNA

		mRNA
Application	For DNA and RNA transfection; recommended for hard-to-transfect cells	For mRNA transfection, enabling high expression in various hard-to-transfect cells	For baculovirus and plasmid DNA transfection in insect cells such as sf9 and High Five	Powder-form PEI transfection reagent for large-scale cell transfection experiments

Cat.NO.	40818ES	40820ES	40821ES	40823ES	40824ES
Product Name	Booster Transfection Reagent	Liposomal Transfection Reagent	siRNA/miRNA Transfection Reagent	mRNA Transfection Reagent	Insect Cell Transfection Reagent
Cell Type	Common cells	Common cells	Common cells	Common cells	Sf9, High Five



Nucleic Acid Type	DNA	DNA	DNA	DNA	DNA




Application	For lentivirus and AAV packaging, as well as protein expression in 293 cells	Universal transfection reagent for viral packaging (suitable for LV and AAV)		Dedicated transfection reagent for AAV viral packaging

Find cell line-specific protocols for cancer cell lines in the table below.

Cell Name	Cell name abbreviation	Nucleic Acid Type
Primary mouse skin fibroblasts	MSFs	DNA
Primary human skin fibroblasts	HSFs	DNA
Primary mouse hepatocytes	PMHs	DNA
Primary mouse neural stem cells	mNSCs	mRNA
Primary human pulmonary artery endothelial cells	HPAECs	DNA
Primary bone marrow–derived macrophages	BMDMs	siRNA
Primary chicken embryo fibroblasts	CEFs	DNA
Primary porcine skeletal muscle satellite cells	PSCs(or PMSCs)	DNA
Primary glioblastoma cells	GBMs	SiRNA
Primary porcine alveolar macrophages	PAMs	DNA

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