Описание
p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein.p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth.
Specifications
|
Nature name |
Nature Details |
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Product Alias |
MHC, HLA-A, P53, TP53, Antigen NY-CO-13, BCC7, FLJ92943, LFS1, TRP53 |
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Species |
Human |
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Expression sequence |
Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and HMTEVVRHC peptide |
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Accession |
A0A140T913(HLA-A*02:01)&P61769(B2M)&HMTEVVRHC |
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Label |
C-His-Avi |
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Expression system |
HEK293 |
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Molecular weight |
The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result. |
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purity |
> 95% as determined by SDS-PAGE and HPLC. |
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Endotoxin |
< 1.0 EU per μg by the LAL method. |
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preparation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally5% trehalose is added as protectant before lyophilization. |
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Appearance |
Lyophilized Powder |
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Biological Activity |
【ELISA Data】Immobilized Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer, His Tag with the EC50 of 44.7ng/ml determined by ELISA. |
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Reconstitution method |
Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.。 |
Product Features
High purity, high activity, low endotoxin, high batch consistency
Application Cases
Product Data
Tris-Bis PAGE
Storage conditions
Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
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Расследование
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