Описание
HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.
Specifications
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Nature name |
Nature Details |
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Product Alias |
HLA0201, MHC I, MAGE-A4, CT1.4, MAGE4A, MAGE4B, MAGE-X2, member 4 |
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Species |
Human |
|
Expression sequence |
Gly25-Thr305(HLA-A*02:01),Ile21-Met119,(B2M) and GVYDGREHTV peptide |
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Accession |
A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV |
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Label |
C-His-Avi |
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Expression system |
HEK293 |
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Molecular weight |
The protein has a predicted MW of 50.5 kDa. Due to glycosylation,the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. |
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purity |
> 95% as determined by SDS-PAGE and HPLC. |
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Endotoxin |
< 1.0 EU per μg by the LAL method. |
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preparation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally5% trehalose is added as protectant before lyophilization. |
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Appearance |
Lyophilized Powder |
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Biological Activity |
【ELISA Data】Immobilized Human MAGE-A4 (HLA-A*02:01) Complex, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MAGE-A4 (HLA-A*02:01) Antibody, hFc Tag with the EC50of 12.7ng/ml determined by ELISA. |
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Reconstitution method |
Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.。 |
Product Features
High purity, high activity, low endotoxin, high batch consistency
Application Cases
Product Data
Tris-Bis PAGE
Storage conditions
Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
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Расследование
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