Описание
AFP (alpha-Fetoprotein), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. It is one of the earliest markers of the hepatocyte lineage. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly.
Specifications
|
Nature name |
Nature Details |
|
Product Alias |
Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-feto, HPAFP, AFPD, FETA, MHC |
|
Species |
Human |
|
Expression sequence |
Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FMNKFIYEI peptide |
|
Accession |
A0A140T913 (HLA-A*02:01)&P61769 (B2M)&FMNKFIYEI |
|
Label |
C-His-Avi |
|
Expression system |
HEK293 |
|
Molecular weight |
Approximately 50.6 kDa.Due to glycosylation, the protein migrates to53-60 kDa based on Tris-Bis PAGE result. |
|
purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
preparation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【ELISA Data】 Immobilized Human AFP (HLA-A*02:01) Complex, His Tag at 2μg/mL(100μL/well) on the plate. Dose response curve for Anti-AFP (HLA-A*02:01) Antibody, hFc Tag with the EC50of 8.2ng/mLdetermined by ELISA. |
|
Reconstitution method |
Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.。 |
Product Features
High purity, high activity, low endotoxin, high batch consistency
Application Cases
Product Data
Tris-Bis PAGE
Storage conditions
Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
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Расследование
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