Description
Product Properties
|
Alternative Names |
BMPR1A, ALK-3, ALK3, ALK 3 |
|
Species |
Canine |
|
Expression Sequence |
Gln24-Arg152 |
|
Accession |
NP_001138622.1 |
|
Tag |
C-hFc |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 40.94 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【Cell Based Assay】Measured by its ability to inhibit rhBMP4-induced alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is typically 0.5 - 2.5 μg/mL in the presence of 50 ng/mL of recombinant human BMP4. |
|
Reconstitution Method |
Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months.
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