Description
Product Properties
|
Alternative Names |
CD221, IGF1R, IGF-1R, IGF-I R, IGF-I receptor, IGFIR, IGFR, JTK13, MGC142170, MGC142172, MGC18216 |
|
Species |
Mouse |
|
Expression Sequence |
Glu31-His936 |
|
Accession |
Q60751 |
|
Tag |
C-His |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 80.72 kDa (alpha subunit) and 23.0 kDa (beta subunit). Due to furin cleavage and glycosylation, the protein migrates to 110-140 kDa(alpha subunit) and 50-65 kDa(beta subunit) based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【ELISA Data】 Immobilized Mouse IGF1R, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 2.3μg/ml determined by ELISA. |
|
Reconstitution Method |
Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months.
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