Description
Product Properties
|
Alternative Names |
Alkaline Phosphatase, ALP-1, Alkaline phosphatase, placental-like, GCAP, PLAP-like, ALPG, ALPPL, ALPPL2 |
|
Species |
Human |
|
Expression Sequence |
Ile20-Asp503 |
|
Accession |
P10696 |
|
Tag |
C-His-Avi |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 55.48 kDa. Due to glycosylation, the protein migrates to 56-70 kDa based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE,> 90% as determined by HPLC |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【ELISA Data】 Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type) , His Tag with the EC50 of 23.4ng/ml determined by ELISA. |
|
Reconstitution Method |
Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
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