Human TGF-alpha Protein, hFc Tag
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Human TGF-alpha Protein, hFc Tag
Human TGF-alpha Protein, hFc Tag
Product Properties Synonyms ETGF; TGF-alpha; TGF type 1; TGFA Species Human Source HEK293 Sequence Val40-Ala89  Accession P01135-1 Tag N-hFc Molecular Weight The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Bis-Tris PAGE and HPLC Activity ELISA Data: Immobilized Human TGF-alpha, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml...

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Human TGF-alpha Protein, hFc Tag

YeasenSKU: 95041ES25
Size: 25 ug
Price:
$185.00
Stock:
In stock

Product Properties

Synonyms

ETGF; TGF-alpha; TGF type 1; TGFA

Species

Human

Source

HEK293

Sequence

Val40-Ala89

Accession

P01135-1

Tag

N-hFc

Molecular Weight

The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Bis-Tris PAGE and HPLC

Activity

ELISA Data: Immobilized Human TGF-alpha, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA

SPR Data: Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 μM as determined in SPR assay.

Formulation

Lyophilized from 0.22μm filtered solution in 50mM Tris,100mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

lyophilized powder

Storage
Store at -25°C to -15°C. The product is valid for 1 year upon receipt.

After reconstitution, store at -85°C to -65°C and use within 3 months.

Reconstitution

Centrifuge before opening the vial. It is recommended to dissolve the lyophilized protein in distilled water. The reconstitution concentration should exceed 100 μg/ml.

Note

1.Please operate with lab coats and disposable gloves, for your safety.

2.This product is for research use only.

Storage

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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.

Certain applications may require additional third-party intellectual property rights.

Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.