Description
Specifications
|
Source |
Biotinylated Human PLAU/uPA Protein (active form) is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ser21-Leu431, which consists of two chains: Long chain A (Ser21-Phe177) and chain B (Ile179-Leu 431). The long chain A is further cleaved to yield a short chain A (Lys156-Phe 177) and N-terminal fragment (Ser21-Lys155).[Accession | P00749-1] |
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Molecular Weight |
The protein has a predicted MW of 49.3 kDa. Due to protein lysis and glycosylation, the protein migrates to 24-26 kDa(long chain A), 35-38 kDa(chain B) and 52-60 kDa(long chain A&chain B) based on Tris-Bis PAGE result. |
|
Endotoxin |
Less than 1EU per ug by the LAL method. |
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Purity |
> 95% as determined by Tris-Bis PAGE and HPLC |
|
Activity |
ELISA Data: Immobilized Human uPAR, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 13.6ng/ml determined by ELISA. |
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Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -25~-15℃ for 1 year from the date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves for your safety.
2.This product is for research use only.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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