Description
|
Product Alias |
sHLA-G,HLA G antigen, b2 microglobulin |
|
Species |
Rhesus macaque |
|
Expression sequence |
Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide |
|
Accession |
O02948(HLA-G)&Q6V7J5(B2M)&RIIPRHLQL |
|
Label |
C-His-Avi |
|
Expression system |
HEK293 |
|
Molecular weight |
The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 58-63 kDa based on SDS-PAGE result. |
|
purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
endotoxin |
< 1.0 EU per μg by the LAL method. |
|
preparation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【ELISA Data】Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 1.4μg/ml determined by ELISA . 【SPR Data】Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag with an affinity constant of 27.18 nM as determined in SPR assay . |
|
Reconstitution method |
Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing. |
Product Features
High purity, high activity, low endotoxin, high batch consistency
Storage
Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months
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