Description
Specifications
Source |
Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Asp18-His259.[Uniprot | P20138-1] |
Molecular Weight |
The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. |
Endotoxin |
Less than 1EU per μg by the LAL method. |
Purity |
> 95% as determined by Tris-Bis PAGE and HPLC |
Activity |
ELISA Data: Immobilized Human Siglec-3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA. |
Formulation |
Supplied as 0.22μm filtered solution in 20mM PB, 500mM NaCl (pH 8.0). |
Storage
The product should be stored at -85~-65℃ for 1 year from date of receipt.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
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