Description
Specifications
Synonyms |
ErbB2; EGFR2;CD340; HER-2 |
Source |
Human Her2/ErbB2 Domain 4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Pro489-Cys630.[Accession | P04626-1] |
Molecular Weight |
The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on SDS-PAGE result. |
Endotoxin |
Less than 1EU per μg by the LAL method. |
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
Activity |
ELISA Data: Immobilized Human Her2 Domain 4, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 13.1ng/ml determined by ELISA. |
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.