Description
|
Product Alias |
MHC, WT1, RMF, AWT1, GUD, NPHS4GUD, WAGR, WIT-2, WT33 |
|
Species |
Human,Mouse |
|
Expression sequence |
Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide |
|
Accession |
A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&P61769(B2M)&RMFPNAPYL |
|
Label |
C-His-Avi |
|
Expression system |
HEK293 |
|
Molecular weight |
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
preparation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【ELISA Data】Immobilized Chimeric HLA-A*02:01 (m汐3) &B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA. |
|
Reconstitution method |
Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing. |
Product Features
High purity, high activity, low endotoxin, high batch consistency.
Storage
Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
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