Description
|
Product Alias |
MHC, MY-ESO-1, CT6.1, LAGE-2, CTAG1, CTAG1B, ESO1CTAG, LAGE2A, NY-ESO-1 |
|
Species |
Human |
|
Expression sequence |
Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQC peptide |
|
Accession |
A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQC |
|
Label |
C-His-Avi |
|
Expression system |
HEK293 |
|
Molecular weight |
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
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preparation |
Lyophilized from 0.22 μm filtered solution in 20mM PB, 500mM NaCl (pH 7.4). Normally 8% mannitol is added as protectant before lyophilization. |
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Appearance |
Lyophilized Powder |
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Biological Activity |
【ELISA Data】Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&NY-ESO-1(SLLMWITQC) Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA . 【SPR Data】Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag with an affinity constant of 0.09 nM as determined in SPR assay |
|
Reconstitution method |
Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing. |
Product Features
High purity, high activity, low endotoxin, high batch consistency.
Storage
Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
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