Recombinant Mouse NGF Protein _ 92101ES

SKU: 92101ES20

Size: 20 μg
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Description

NGF was discovered as a molecule that promoted the survival and differentiation of sympathetic and sensory neurons in the peripheral nervous system.NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4).

 

Product Properties

Synonyms

beta Nerve Growth Factor; Ngfb

Uniprot No.

NP_001106168.1

Source

Recombinant Mouse NGF Protein is expressed from CHO Stable Cells Cells with an initial Met at the C-terminus. It contains Ser 122-Gly 241.

Molecular Weight

The recombinant mouse NGF has a calculated molecular mass of 13.5 kDa as estimated in SDS-PAGE under reducing conditions.

Biological Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-6 ng/mL.

Purity

> 95% as determined by SDS-PAGE.

Endotoxin

<1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from sterile 150mM NaCl, 50mM NaAc, pH 5.5.Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. 

 

Shipping and Storage

The product should be stored at -25~-15℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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