Recombinant Rat Platelet-derived Growth Factor-AA (Rat PDGF-AA) _ 91604ES

SKU: 91604ES10

Size: 10 μg
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Sale price$190.00

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Description

PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The A-chain homodimers of the platelet-derived growth factor (PDGF AA) are widely expressed in normal and transformed cells. The mitogenic properties of PDGF AA are well established. FGF-2 and platelet-derived growth factor-A (PDGF-AA) are potent modulators of oligodendrocytes, the main responsible cells for myelin regeneration. PDGF has been suggested to have a significant role in wound healing. 

 

Product Properties

Synonyms

PDGF1, PDGF-A, PDGFAA, PDGF-AA

Accession

P28576

GeneID

25266

Source

E.coli-derived rat PDGF-AA, Ser87-Arg196, with an N-terminal Met.

Molecular Weight

Approximately 25.3 kDa.

AA Sequence

MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

>98% by SDS-PAGE analyses.

Biological Activity

The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0 ng/mL, corresponding to a specific activity of > 2.0 × 105 IU/mg. Fully biologically active when compared to standard.

Endotoxin

0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

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