Recombinant Human FGF basic/FGF2/bFGF, GMP Protein
Recombinant Human FGF basic/FGF2/bFGF, GMP Protein
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Recombinant Human FGF basic/FGF2/bFGF, GMP ProteinRecombinant Human FGF basic/FGF2/bFGF, GMP Protein

Recombinant Human FGF basic/FGF2/bFGF, GMP Protein

YeasenSKU: 91341ES10
Size: 10 μg
Price:
$157.00

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Description

Basic fibroblast growth factor (bFGF) is a member of the fibroblast growth factor (FGF) family, which is a growth factor and signaling protein encoded by the FGF2 gene. FGF family members have a wide range of activities that promote mitosis and cell survival, and are involved in various biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. In normal tissues, bFGF exists in the extracellular matrix beneath the basement membrane and vascular endothelium. bFGF can stimulate and regulate the differentiation and proliferation of various cells originating from the mesoderm and neuroectoderm, including vascular endothelial cells, epithelial cells, myoblasts, osteoblasts, and glial cells. FGF-2 plays an important role in embryonic development and tissue healing. bFGF is the fundamental component that maintains pluripotency in all embryonic and induced pluripotent stem cell culture media.

The product is expressed in E. coli and provided in liquid form, with high activity, high purity and low endotoxin.

Product Properties

Synonyms

bFGF, FGF basic, FGF2, FGF-2, fibroblast growth factor 2 (basic), HBGF-2, Prostatropin.

Source

Human bFGF Protein is expressed from E.coli without tag.

Sequence

Ala 144 - Ser 288.

Molecular Weight

Approximately 16.29 kDa.

Accession

P09038.

Biological Activity

Human bFGF GMP Protein stimulates proliferation of murine balb/c 3T3 cells.  The specific activity of Human bFGF GMP Protein is >8.0 x 10^5 IU/mg, which is calibrated against Basic Fibroblast Growth Factor WHO International Standard (NIBSC code: 90/712).

Purity

＞95% as determined by SDS-PAGE and HPLC.

Endotoxin

＜0.01 EU/μg protein as determined by LAL method.

Sterility

Negative.

Mycoplasma

Negative.

Vitro Virus

Negative.

Host Cell Protein

Less than 0.5 ng/μg when tested by ELISA.

Host Cell DNA

Less than 20 ng/mg when tested by qPCR.

Formulation

Liquid from 0.22 μm filtered solution in PBS.

Physical Appearance

Liquid.

Storage

The product should be stored at -85~-65℃ for 2 years.

Notes

1.Please operate with lab coats and disposable gloves, for your safety.

2.This product is for research use only.

Assay Data

SDS-PAGE

Figure 1. The purity is greater than 95% as determined by SDS-PAGE under reduced condition.

Figure 1. The purity is greater than 95% as determined by SDS-PAGE under reduced condition.

SEC-HPLC

Figure 2. The purity is greater than 95% as determined by SEC-HPLC.

 Figure 2. The purity is greater than 95% as determined by SEC-HPLC.

Cell Based Assay

Figure 3. Human bFGF GMP Protein stimulates proliferation of murine balb/c 3T3 cells. The specific activity of Human bFGF GMP Protein is >8.0 x 10^5 IU/mg, which is calibrated against Basic Fibroblast Growth Factor WHO International Standard (NIBSC code: 90/712).

Figure 3. Human bFGF GMP Protein stimulates proliferation of murine balb/c 3T3 cells. The specific activity of Human bFGF GMP Protein is >8.0 x 10^5 IU/mg, which is calibrated against Basic Fibroblast Growth Factor WHO International Standard (NIBSC code: 90/712).

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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.

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