Description
Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family. Based on its structure, FGF-21 is further classified into a subfamily of FGFs along with FGF-19 and -23. At the amino acid sequence level, mature rat FGF-21 is 92% identical to mouse FGF-21. In comparison to other FGF subfamilies, a heparin-binding domain is uniquely absent in FGF-19 subfamily members. Lack of this domain confers endocrine function to FGF-19 members and enables them to freely diffuse within tissues and accumulate in the circulatory system. The biological activity of FGF-21 requires binding to Klotho beta, a co-receptor that is in complex with cell surface FGF receptors (FGF R). Binding of FGF-21 to Klotho beta facilitates FGF R activation and autophosphorylation resulting in the initiation of multiple downstream signaling cascades. FGF-21 cannot independently bind to FGF Rs, thus its effects are restricted to target tissues that express Klotho beta. FGF-21 functions as a physiological regulator of cellular metabolism, including glucose uptake in adipocytes and cellular sensitivity to insulin. FGF-21 is basally expressed in the pancreas, thymus, and liver, as well as in adipose tissue. Local and systemic metabolic stress has been shown to induce expression of FGF-21 in the liver, muscles, and fat. Modulation of FGF-21 expression is associated with a number of metabolic disorders, including obesity and diabetes.
Product Properties
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Synonyms |
FGF21; FGF-21; fibroblast growth factor 21 |
|
Accession |
Q8VI80 |
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GeneID |
170580 |
|
Source |
E.coli-derived Rat FGF-21, Ala29-Ser208. |
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Molecular Weight |
Approximately 19.7 kDa. |
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AA Sequence |
AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS |
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Tag |
None |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Purity |
> 95% by SDS-PAGE and HPLC analyses. |
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Biological Activity |
The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/mL, corresponding to a specific activity of > 2.0 × 103 IU/mg in the presence of 5 µg/mL of rMuKlotho-β and 10 μg/mL of heparin. Fully biologically active when compared to standard. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, 500 mM NaCl, pH 7.4. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!