Description
Description
Monocyte colony-stimulating factor (M-CSF) is a macrophage lineage-specific growth factor. M-CSF Induces vascular endothelial growth factor production and angiogenic activity from human monocytes. Whatmore, M-CSF and GM-CSF are mediators involved in regulating the numbers and function of macrophage lineage populations and have been shown to contribute to macrophage heterogeneity.
Product information
|
Synonyms |
CSF-1, MGI-IM, MCSF, M-CSF, MGC31930 |
|
Uniprot No. |
|
|
Source |
Recombinant Mouse M-CSF is expressed from Yeast with N-His. It contains Lys33-Glu262. |
|
Molecular Weight |
The recombinant mouse CSF1 comprises 230 amino acids and has a predicted molecular mass of 26 kDa. The apparent molecular mass of the protein is approximately 30 kDa in SDS-PAGE under reducing conditions due to glycosylation |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
<1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4,with 1%BSA. |
|
Reconstitution |
1. We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS, pH 7.4, with 0.1% BSA to a concentration of 0.1-1.0 mg/mL. 2.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. 3.Further dilutions should be made in PBS, which must contain carrier proteins, such as 0.1% BSA, 10% FBS, 5% HSA, 5% trehalose, one of four options. |
Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Notes
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.