Description
Interleukin-6 (IL-6) is a pleiotropic cytokine that plays a critical role in host defense by modulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells, and keratinocytes, IL-6 exerts diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces the expression of hepatic acute-phase proteins, and regulates bone metabolism. Signaling is transduced through the IL-6 receptor system, which comprises two chains: IL-6Rα and gp130. This product is recombinant human IL-6 supplied as a lyophilized powder with high activity, high purity, and low endotoxin.
Product Information
|
Product Name |
Catalog No. |
Size |
|
Recombinant Human IL-6 Protein |
90107ES08 |
5 μg |
|
90107ES20 |
20 μg |
|
|
90107ES50 |
50 μg |
|
|
90107ES60 |
100 μg |
|
|
90107ES80 |
1 mg |
Specifications
|
Synonyms |
Interleukin-6, BSF2, HSF, IFNB2, IL6 |
|
Source Species |
Human |
|
Expression Sequence |
Val30-Met212 |
|
Accession |
P05231 |
|
Tag |
No Tag |
|
Expression System |
E. coli |
|
Molecular Weight |
Approximately 20.8 kDa. |
|
Purity |
> 95% as determined by SDS-PAGE |
|
Endotoxin |
< 0.01 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is 0.1–0.8 ng/mL. |
|
Reconstitution |
Centrifuge the vial before opening to bring contents to the bottom. Reconstitute with sterile distilled water or PBS to a concentration of 0.1–1.0 mg/mL, and dilute further as needed for subsequent experiments. For long-term storage, a carrier protein (0.1% BSA) is recommended in the dilution buffer. Aliquot into single-use volumes and store at –80°C; avoid repeated freeze-thaw cycles. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Application
SDS-PAGE
Figure 1. Human IL-6 Protein on SDS-PAGE under reduced (R) & Non-reducing (NR) condition. The purity is greater than 95%.
Bioactivity
Figure 2. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is 0.1–0.8 ng/mL.
Storage
Store at –25 to –15°C. Valid for 1 year from the date of receipt. After reconstitution, store under sterile conditions at 2–8°C for up to 7 days, or at –85 to –65°C for up to 3 months.
Documents:
Safety Data Sheet
Manuals:
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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