Description
IL-6 is similar to other interleukins in that it has a compact globular folding structure and is held in place by two disulfide bonds. The cysteine involved in forming these two disulfide bonds is highly conserved. IL-6, expressed by a variety of cells, regulates cell growth and differentiation in various tissues and is an important pro-inflammatory and immunomodulatory cytokine.
Product Properties
Synonyms |
Interleukin-6,BSF2,HSF,IFNB2 |
Uniprot No. |
P05231 |
Source |
Recombinant Human IL-6 Protein is expressed from E.coli without tag. It contains Val30-Met212. |
Molecular Weight |
The protein has a predicted 20.8 kDa same as Tris-Bis PAGE result. |
Purity |
> 95% as determined by SDS-PAGE. |
Biological Activity |
The ED50 as determined by a cell proliferation assay using human TF-1 cells is 0.1-0.5 ng/mL. |
Endotoxin |
< 1 EU per 1μg of the protein by the LAL method. |
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS. Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Shipping and Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3-6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.