Recombinant Human IL-6 Protein _ 90107ES

SKU: 90107ES08

Size: 5 μg
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Description

IL-6 is similar to other interleukins in that it has a compact globular folding structure and is held in place by two disulfide bonds. The cysteine involved in forming these two disulfide bonds is highly conserved. IL-6, expressed by a variety of cells, regulates cell growth and differentiation in various tissues and is an important pro-inflammatory and immunomodulatory cytokine.

 

 Product Properties

 

Synonyms

Interleukin-6,BSF2,HSF,IFNB2

Uniprot No.

P05231

Source

Recombinant Human IL-6 Protein is expressed from E.coli without tagIt contains Val30-Met212.

Molecular Weight

The protein has a predicted 20.8 kDa same as Tris-Bis PAGE result.

Purity

> 95% as determined by SDS-PAGE.

Biological Activity

The ED50 as determined by a cell proliferation assay using human TF-1 cells is 0.1-0.5 ng/mL.  

Endotoxin

< 1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS. Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

Shipping and Storage

 

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.  

3-6 months, -85~-65℃ under sterile conditions after reconstitution.  

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

 Cautions

 

1.Please operate with lab coats and disposable gloves,for your safety.   

2.This product is for research use only.

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