DMSO is a non-protic polar solvent, commonly used in chemical reactions, PCR reactions, and as a vitrification cryoprotective agent for the preservation of cells, tissues, and organs. DMSO is used in cell freezing culture media to protect cells from mechanical damage caused by ice crystals. It can also be used for the cryopreservation of a series of cell lines such as primary culture, sub-culture, recombinant allopolyploid, hybridoma, embryonic stem cells (ESCs), and hematopoietic stem cells. Additionally, it is often mixed with BSA or FBS

Specification

Components

Storage

This product should be stored at RT for 2 years.

Features

Excellent compatibility: Fully compatible with LDPE, HDPE, PP, PPCO, PMP, nylon, and Teflon FEP.

Broad solubility: Miscible with water, ethanol, acetone, ether, benzene, and chloroform.

Moisture sensitive: Hygroscopic; protect from humidity.

High thermal stability: Stable in acidic, basic, and neutral solutions up to 150 °C with minimal purity loss.

Reversible solidification: May solidify at room temperature but easily re-melts without degradation.

Application

Cell culture: Solvent for drugs and reagents in cell-based experiments.

Cell cryopreservation: Major component of freezing media that protects cells from freeze–thaw damage.

Biochemistry: Used to dissolve and dilute bioactive molecules.

Pharmacology: Solvent and carrier for studying drug permeability and bioactivity.

Documents:

Safety Data Sheet

60313_MSDS_HB251104_EN.PDF

Manuals

60313_Manual_Ver.EN20251104.pdf

Published References

[1] Yao W, Liu C, Wang N, Zhou H, Chen H, Qiao W. An MRI-guided targeting dual-responsive drug delivery system for liver cancer therapy. J Colloid Interface Sci. 2021;603:783–798. doi:10.1016/j.jcis.2021.06.151 (IF: 8.128)

[2] Liu X, Zhan T, Gao Y, et al. Benzophenone-1 induced aberrant proliferation and metastasis of ovarian cancer cells via activated ERα and Wnt/β-catenin signaling pathways. Environ Pollut. 2022;292(Pt B):118370. doi:10.1016/j.envpol.2021.118370 (IF: 8.071)

[3] Gao Z, Liu S, Tan L, et al. Testicular toxicity of bisphenol compounds: Homeostasis disruption of cholesterol/testosterone via PPARα activation. Sci Total Environ. 2022;836:155628. doi:10.1016/j.scitotenv.2022.155628 (IF: 7.963)

[4] Wu H, Zhan T, Cui S, et al. Endothelial barrier dysfunction induced by anthracene and its nitrated or oxygenated derivatives at environmentally relevant levels. Sci Total Environ. 2022;802:149793. doi:10.1016/j.scitotenv.2021.149793 (IF: 7.963)

[5] Lu L, Wu H, Cui S, et al. Pentabromoethylbenzene exposure induces transcriptome aberration and thyroid dysfunction: In vitro, in silico, and in vivo investigations. Environ Sci Technol. 2020;54(19):12335–12344. doi:10.1021/acs.est.0c03308 (IF: 7.864)

[6] Sun J, Guo Y, Fan Y, Wang Q, Zhang Q, Lai D. Decreased expression of IDH1 by chronic unpredictable stress suppresses proliferation and accelerates senescence of granulosa cells through ROS-activated MAPK signaling pathways. Free Radic Biol Med. 2021;169:122–136. doi:10.1016/j.freeradbiomed.2021.04.016 (IF: 7.376)

[7] Zhan T, Zhang L, Cui S, Liu W, Zhou R, Zhuang S. Dioxybenzone triggers enhanced estrogenic effect via metabolic activation: In silico, in vitro and in vivo investigation. Environ Pollut. 2021;268(Pt B):115766. doi:10.1016/j.envpol.2020.115766 (IF: 6.793)

[8] Zhou X, Zhang Z, Jiang W, et al. Naringenin is a potential anabolic treatment for bone loss by modulating osteogenesis, osteoclastogenesis, and macrophage polarization. Front Pharmacol. 2022;13:872188. doi:10.3389/fphar.2022.872188 (IF: 5.811)

[9] Zhi W, Li S, Wan Y, Wu F, Hong L. Short-term starvation synergistically enhances cytotoxicity of Niraparib via Akt/mTOR signaling pathway in ovarian cancer therapy. Cancer Cell Int. 2022;22(1):18. doi:10.1186/s12935-022-02447-8 (IF: 5.722)

[10] Li C, Li J, Li Y, et al. Isorhamnetin promotes MKN-45 gastric cancer cell apoptosis by inhibiting PI3K-mediated adaptive autophagy in a hypoxic environment. J Agric Food Chem. 2021;69(29):8130–8143. doi:10.1021/acs.jafc.1c02620 (IF: 5.279)

[11] Wu R, Zhang H, Zhao M, et al. Nrf2 in keratinocytes protects against skin fibrosis via regulating epidermal lesion and inflammatory response. Biochem Pharmacol. 2020;174:113846. doi:10.1016/j.bcp.2020.113846 (IF: 4.960)

[12] Zheng Y, Luo A, Liu X. The imbalance of mitochondrial fusion/fission drives high-glucose-induced vascular injury. Biomolecules. 2021;11(12):1779. doi:10.3390/biom11121779 (IF: 4.879)

[13]Qiao X, Yang Y, Huang R, et al. E-Jet 3D-printed scaffolds as sustained multi-drug delivery vehicles in breast cancer therapy. Pharm Res. 2019;36(12):182. doi:10.1007/s11095-019-2687-3

 (IF: 3.896)

[14]Dong X, Bai Y, Xu Z, et al. Phlorotannins from Undaria pinnatifida sporophyll: Extraction, antioxidant, and anti-inflammatory activities. Mar Drugs. 2019;17(8):434. doi:10.3390/md17080434 (IF: 3.772)

[15]Pan Z, Chen Q, Zheng X, et al. JuBei oral liquid induces mitochondria-mediated apoptosis in NSCLC cells. Onco Targets Ther. 2020;13:7585–7598. doi:10.2147/OTT.S254464 (IF: 3.337)

[16] Bai Y, Sun Y, Gu Y, Zheng J, Yu C, Qi H. Preparation, characterization and antioxidant activities of kelp phlorotannin nanoparticles. Molecules. 2020;25(19):4550. doi:10.3390/molecules25194550 (IF: 3.267)

[17] Feng H, Tang J, Zhang P, Miao Y, Wu T, Cheng Z. Anti-adipogenic 18,19-seco-ursane stereoisomers and oleane-type saponins from Ilex cornuta leaves. Phytochemistry. 2020;175:112363. doi:10.1016/j.phytochem.2020.112363 (IF: 3.044)

[18]Li S.S., Jiang W.L., Xiao W.Q., et al. KMT2D deficiency enhances the anti-cancer activity of L48H37 in pancreatic ductal adenocarcinoma. World J Gastrointest Oncol. 2019;11(8):599–621. doi:10.4251/wjgo.v11.i8.599 (IF: 2.758)

[18] Liu Y, Chen G, Wang B, Wu H, Zhang Y, Ye H. Silencing circRNA protein kinase C iota (circ-PRKCI) suppresses cell progression and glycolysis of human papillary thyroid cancer through circ-PRKCI/miR-335/E2F3 ceRNA axis. Endocr J. 2021;68(6):713–727. doi:10.1507/endocrj.EJ20-0726 (IF: 2.349)

[19] Deng X.L., Zheng R.R., Han Z.Z., Gu L.H., Wang Z.T. New chlorophenolic glycoside from Curculigo orchioides and their activities on 5α-reductase. J Asian Nat Prod Res. 2021;23(4):333–340. doi:10.1080/10286020.2020.1731800 (IF: 1.345)

[21]Li L, Peng W, Tian X. Protective effects and mechanisms of microRNA-182 on oxidative stress in RHiN. Open Life Sci. 2019;14:400–409. doi:10.1515/biol-2019-0045 (IF: 0.504)