Frequently Bought Together
This product is a Linear Polyethylenimine (PEI) after optimization and transformation, with a concentration of 1 mg/mL. This product is purely chemically synthesized and does not contain animal-derived ingredients. It is particularly suitable for co-transfection of multiple plasmids, for the production of recombinant virus vectors and the instantaneous expression of recombinant proteins. This product has low cytotoxicity, high transfection efficiency, and is compatible with antibiotics. It has high gene expression efficiency in HEK293 and other cells.
Feature
High Transfection Efficiency: Exhibits excellent transfection efficiency across a variety of cell lines, with particularly high gene expression levels in cells such as HEK293.
Low Cytotoxicity: Demonstrates minimal cellular toxicity, allowing transfected cells to maintain high viability and normal morphology.
Suitable for Multi-Plasmid Co-transfection: Particularly well-suited for the co-transfection of multiple plasmids, making it ideal for the production of recombinant viral vectors (such as LV and AAV) and the transient expression of recombinant proteins.
Purely Chemically Synthesized & Animal-Origin Free: Features high product purity, eliminating the potential biosafety risks associated with animal-derived components.
Application
Cell Transfection
Specification
| Physical and chemical Properties | Information |
| Form | Liquid |
| Serum Compatible | Yes |
| Cell Type | Established Cell Lines |
| Sample Type | Plasmid DNA |
| Transfection Technique | PEI-Based Transfection |
Components
|
Components No. |
Name |
40820ES01 |
40820ES04 |
40820ES10 |
40820ES60 |
|
40820 |
Hieff Trans™ PEI Transfection Reagent |
100 uL |
1.5 mL |
10 mL |
100 mL |
Figures
1. Quality
| Item |
Specifications |
R&D |
GMP |
|
Clarity |
Colorless liquid |
√ |
√ |
|
Activity |
Transfection efficiency>70% Cell viability>80% |
√ |
√ |
|
Endotoxin |
<0.5 EU/mL |
√ |
√ |
|
Sterility |
Sterile after 14 days |
√ |
√ |
|
Mycoplasma |
Absence |
√ |
√ |
|
pH |
<7.0 |
|
√ |
|
Osmolality |
≤30 mOsm/kg |
|
√ |
|
Impurity distribution |
No residual acetonitrile, methanol, ether, toluene |
|
√ |
2. Transfection Efficiency
Figure 1. The transfection efficiency of Hieff Trans™ PEI Transfection Reagent-GMP.
Hieff Trans™ PEI Transfection Reagent-GMP was used to transfect the suspension cell HEK293F cell line and adherent cell HEK293T cell line in a 6-well format. AAV-GFP expression was analyzed 48 hours post-transfection. (the ratio of the plasmid to reagent is 1:1)
3. Customer's test data (AAV2)
|
Suspended cell 293F |
|||
|
Sample |
Transfection efficiency |
Cell viability |
Cell count |
|
YEASEN |
100% |
92.00% |
6.80E+6 /ml |
|
P* |
100% |
71.00% |
6.05E+6 /ml |
Culture vessel: 30 mL medium (125 mL shake flask)
Medium: OPM-293CD-05 serum-free medium
Incubation time: 72 h
Plasmid: 60 μg(MCS-GFP : RC2 : pHelper = 1 : 1 : 1 = 20 μg : 20 μg : 20 μg)
Transfection reagent: 120 -180 μL
4. Customer's test data (LV)
Cell viability: 98%
Culture vessel: 50 mL medium (250 mL shake flask)
Cell count: 5E+6/mL
Plasmid: 2 mg/L
Transfection reagent: 4-8 ml/L
Incubation time: 48 h
Storage
FAQ
Citations
Documents
| Manual |
|---|
| https://seas.ysbuy.com/prod/doc/mainDoc/40820_Manual_HB230215_EN.pdf |
Applications
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Inquiry
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.