Polybrene (hexadimethrine bromide), also known as Polybrene, is a cationic polymer commonly used in DNA transfection experiments with mammalian cells to enhance the transfection efficiency of liposomes. It is widely employed in retrovirus-mediated gene transfection and lentivirus-mediated gene transfection. The mechanism of action may involve neutralizing the sialic acid on the cell surface, facilitating adsorption by overcoming electrostatic repulsion with virus particles. Polybrene is also recognized as an anticoagulant (heparin antagonist) and is frequently used in the production of non-specifically aggregated red blood cells. Additionally, in protein sequencing, low doses of Polybrene have been shown to significantly improve the degradation of peptides in automated sequencing analysis. The addition of Polybrene to PVDF membranes can also enhance membrane affinity.

Features

• Enhances transduction efficiency: Polybrene, a cationic polymer, neutralizes surface charges between viral particles (e.g., lentivirus) and cells, promoting viral adhesion and significantly boosting infection rates.
• Excellent stability: Stable when stored at 2–8°C with a long shelf life, ensuring consistent performance.
• Simple to use: Ready-to-use—just add directly to virus-containing medium at the recommended ratio, without complex preparation.

Components

Storage

This product should be stored at -25~-15℃ for 2 years.

Application

Cell Transfection; Retroviral Infection

FAQ

Q: The concentration of Polybrene is 10 mg/mL. How should it be diluted?

A: Polybrene is not diluted by preparing a separate diluted solution. Instead, dilution refers to the final working ratio in the experimental system.

For example, if the total reaction volume is 10 mL and a 1:1000 working ratio is required, simply add 10 μL of the 10 mg/mL Polybrene stock solution directly to the experimental system.

Documents:

Safety Data Sheet

40804_MSDS_HB260113_EN.PDF

Manuals

40804_Manual_Ver.EN20260113.pdf

FAQ

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