Hieff Trans™ Liposomal Transfection Reagent is a versatile liposome transfection reagent, suitable for DNA, RNA, and oligonucleotide transfection, with high transfection efficiency for most eukaryotic cells. Its unique formula allows it to be added directly to the medium, and the presence of serum does not affect transfection efficiency, which reduces the damage to cells caused by serum removal. There is no need to remove the nucleic acid-Hieff Trans™ complex or replace it with a fresh medium after transfection, and it can also be removed after 4-6 hours.

Hieff Trans™ is supplied in sterile liquid form. Usually, for 24-well plate transfection, about 1.5 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections; for a 6-well plate, about 6 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections. 160 transfections.

Feature

High Efficiency: Enables transfection in most eukaryotic cells, achieving over 90% efficiency in common cell lines.

Low Toxicity: Transfected cells maintain healthy morphology while expressing abundant target protein.

Easy to Use: Lipid complexes can be directly added to serum-containing culture media.

Versatile Applications: Suitable for both transient and stable transfection experiments.

Application

Cell Transfection

Specification

Components

Figures

1. High transfection efficiency

Figure 1. Efficient DNA transfection in 293 cells.

Transient transfection was performed in 12-well plates using a total of 1 μg plasmid (0.5 μg GFP + 0.5 μg RFP) with 3 μL of transfection reagent.

Cited from: Communications Biology volume 5, Article number: 1296 (2022)

2. High transfection siRNA Transfection

Figure 2. Efficient AMPK gene silencing in HK2 cells.

HK2 cells were transfected in 6-well plates with 100 pmol siRNA targeting AMPK using 6 μL of transfection reagent. High silencing efficiency (+++) was observed with no detectable cytotoxicity

Each reagent was used to transfect the target cell line（Hela cell line in Figure 1a, DF-1 cell line in Figure 1b）in a 96-well format. GFP expression was analyzed 48 hours posttransfection. Hieff Trans™ Liposome Transfection Reagent provided higher GFP transfection efficiency than the competitive brand products.

3.

Figure 3. Comparison of transfection efficiency between Hieff Trans™ Liposome Reagent and other brands.

(A).Hieff Trans™ and supplier P* were used to transfect HeLa and DF-1 cells in 96-well plates. GFP expression was measured 48 hours post-transfection. Hieff Trans™ consistently achieved higher GFP transfection efficiency than the other products.

4. Validated Cell Lines

Figure 4. List of cell lines successfully transfected with Hieff Trans™ Liposomal Transfection Reagent (Under continuous update).

5.Case