Collagenase is a protease and an endopeptidase that specifically recognizes the Pro-X-Gly-Pro sequence—highly abundant in collagen but rarely found in other proteins—and cleaves the peptide bond between the neutral amino acid (X) and glycine (Gly). While many proteases can hydrolyze single-stranded, denatured collagen peptides, collagenase is the only enzyme capable of degrading native collagen fibers with their characteristic triple-helical structure, which are widely present in connective tissues.

This product is derived from Clostridium histolyticum and is a crude enzymatic extract. It contains collagenase (more accurately termed clostridiopeptidase A), which degrades native collagen and reticular fibers, as well as additional proteases, polysaccharidases, and lipases. These auxiliary enzymes effectively hydrolyze other proteins, polysaccharides, and lipids in the extracellular matrix of both connective and epithelial tissues, making this preparation highly suitable for tissue dissociation.

Commercially available bacterial collagenases are classified into four types—Type I, II, III, and IV—primarily based on differences in enzymatic activity profiles, each with distinct application preferences:

Type I Collagenase: Contains balanced activities of collagenase, caseinase, clostripain, and trypsin-like enzymes. Commonly used for the isolation of epithelial cells and cells from liver, lung, adipose tissue, and adrenal glands.

Type II Collagenase: Exhibits higher clostripain activity. Typically used for preparing cells from heart, bone, skeletal muscle, thymus, and cartilage.

Type III Collagenase: Features lower overall protease activity. Often used for mammary cell isolation.

Type IV Collagenase: Contains low trypsin-like activity. Preferred for pancreatic islet cell isolation or any application requiring preservation of cell surface receptor integrity.

This product is Type II Collagenase, with an activity of ≥125 U/mg solid, and is suitable for dissociation of tissues and cells from heart, thyroid, salivary gland, liver, bone, and cartilage.

Specifications

Components

Storage

Store at 4 °C protected from light; stable for 2 years. Stock solutions should be aliquoted and stored at –20 °C protected from light.

Documents:

Safety Data Sheet

40508_MSDS_HB251222_EN.PDF

Manuals

40508_Manual_Ver.EN20251222.pdf

Reference

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