Description
Dibasic endoprotease (also known as Kex2 protease, YSCF protease, EC 3.4.21.61) is responsible for processing the precursors of killer toxin and alpha-factor in yeast. It is a calcium ion-dependent serine protease that specifically recognizes and cleaves peptide bonds at the carboxyl terminus of dibasic amino acid pairs such as Arg-Arg and Lys-Arg.
Kex2 protease cleaves the carboxyl terminus of the second basic amino acid at the RR site in the substrate. The Dibasic Endoprotease Activity Assay Kit uses the synthetic substrate Boc-QRR-pNA (Cat#20820ES) containing a specific peptide-pNA design. This substrate has no absorbance at 405 nm. When Kex2 protease cleaves at the RR site, two fragments are produced: Boc-Gln-Arg-Arg and pNA. pNA is deprotonated in alkaline buffer to form p-nitrophenolate (bright yellow, maximum absorbance at 405 nm). Within a certain concentration range, the rate of change in absorbance at 405 nm is positively correlated with enzyme activity, allowing direct quantification of Kex2 catalytic activity.
The Dibasic Endoprotease Activity Assay Kit is a colorimetric-based dedicated detection tool for Kex2 activity, which can be used for rapid and accurate activity quantification in enzyme preparation R&D, production quality control, and applied research.
Product Information
|
Component No. |
Component Name |
20383ES50 (50 T) |
|
20383-A |
Kex2 Substrate (Boc-QRR-pNA) |
3.3 mg |
|
20383-B |
Kex2 Assay Buffer |
60 mL |
Properties
|
Cat# |
Component Name |
20383ES50 (50T) |
|
20383-A |
Kex2 Substrate (Boc-QRR-pNA) |
3.3 mg |
|
20383-B |
Kex2 Assay Buffer |
60 mL |
Application
Figure 1. Kex2 activity assay kinetic curve.
Storage
Store at -25 to -15 degrees C. The unopened kit is valid for 1 year; after opening, valid for 6 months.
Documents:
Safety Data Sheet
Manuals:
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
You may also like
Inquiry
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.