GoldBand™ 3-color High Range Protein Marker (10-245 KDa ) _ 20352ES

This product consists of 12 highly purified and prestained proteins ranging in molecular weight from 10 kDa to 245 kDa, among them, 72 kDa is orange band, 25 kDa is green band, 10/15/20/35/ 45/60/100/140/180/245 kDa are blue bands, the protein concentration of each band was about 0.1-0.4 mg/mL. Using this product, the state of protein 

electrophoresis and the effect of membrane transfer can be dynamically observed. After SDS-PAGE electrophoresis,

the color bands were transferred to PVDF membrane and NC membrane. This product is conveniently packaged 

and is a ready-to-use product, do not heat, dilute or add reducing agents!

After the prestained protein is combined with the dye, the molecular weight will change in the gel of different 

concentrations. This product is only for reference when judging the molecular weight of the target protein.Components

Storage

The product should be stored at -25℃ ~ -15℃ for two years.For regular use, it can be placed at 4℃, valid for three months. It is recommended to store in aliquots to avoid repeated freezing and thawing!

Stock solution composition

62.5 mM Tris-H₃PO₄(pH 7.5), 2 mM EDTA, 2% (W/V) SDS, 33% (W/V) Glycerol, 5 mM DTT, 0.02% (V/V) proclin300

Instructions

1. After the product is thawed at room temperature,mix gently to fully dissolve the precipitate. 

2. Then take an appropriate amount of this product into the gel hole. mini-gel: 3-5 μL;  when the thickness of the gel is greater than 1.5 mm or the macromolecular bands need to be deepened, the loading volume can be appropriately increased: 5-10 μL.

Notes

1. The product needs to be returned to room temperature before use to fully dissolve the precipitate. Incomplete protein denaturation at low temperature may lead to different degrees of dispersion of electrophoresis bands.

2. In western blot experiments, large proteins (>100 kDa) in the product may require longer transfer times or higher transfer voltages to complete transfer.

3. This product contains SDS, and the protein has been denatured, so it should not be used as a molecular weight reference standard for natural protein molecular electrophoresis.

4. The product will have deviations in protein size under different electrophoresis conditions, but after they are calibrated by non-prestained protein standards in the same buffer system, they can be used for protein determination of similar molecular weights.

5. At low concentration of gel, low molecular weight protein will swim on the dye front.

6. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents!

7. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety！

8. For research use only!

Figure1. 4-20% SDS-PAGE electrophoresis results

Attached table Under different electrophoresis buffer conditions, each band of this product indicates molecular weight

 Manuals