Description
This product consists of 9 highly purified and prestained proteins ranging in molecular weight from 25 kDa to 300 kDa (25,45,72,100,130,160,200,250,300 kDa), among them, 72 kDa is an orange band, 25 kDa is a green band. The labeled apparent molecular weight was calibrated by the molecular weight Marker of standard non-prestained proteins. Using this product, the state of protein electrophoresis and the effect of membrane transfer can be dynamically observed. After SDS-PAGE electrophoresis, the color bands were transferred to the o PVDF membrane and NC membrane. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents! After the prestained protein is combined with the dye, in different buffer systems, there is displacement. There are signs in the instructions, this product is only for reference when judging the molecular weight of the target protein.
Features
- Bright color - three color predye, each strip average concentration of 2.5 μg
- Lower amount of this product into the gel hole - usually used for loading 5-10 μL each time
- Stable performance - 50℃ for 20 h, no degradation
- Long shelf life - can be stored at -30℃ ~ -15℃ for two years
Applications
- Protein migration is monitored during SDS-PAGE electrophoresis
- Monitor protein transfer to the membrane after Western blotting
- Protein size analysis on SDS-PAGE and Western blotting
Specifications
| Product Type Specs | Protein Ladder |
| Size Range | 25 kDa to 300 kDa |
| Number of Markers | 9 |
| Molecular Weight | 25,45,72,100,130,160,200,250,300 kDa |
| Stain Type | 72 kDa is orange band, 25 kDa is green band, the rest are blue bands |
| Storage Buffer | 62.5 mM Tris-H3PO4 (pH 7.5), 2 mM EDTA, 2% (W/V) SDS, 33% (W/V) Glycerol, 5 mM DTT, 0.02% (V/V) proclin300 |
| Ready to Load | ready-to-use product, do not heat, dilute or add reducing agents |
Components
| Components No. | Name | 20347ES76 | 20347ES90 |
| 20347 | GoldBand™ Plus 3-color High Range Protein Marker (25-300 kDa) | 2×250 μL | 10×250 μL |
Storage
The product shouldbe stored at -25℃ ~ -15℃ for two years. For regular use, it can be placed at 4℃, valid for three months. It is recommended to store in aliquots to avoid repeated freezing and thawing!
Figures
Figure 1 7% SDS-PAGE electrophoresis results
Figure 2 Under different electrophoresis buffer conditions, the position of each band of this product