GoldBand™ 3-color Low Range Protein Marker (2.7-40 KDa ) _ 20344ES

This product consists of 9 highly purified and prestained proteins ranging in molecular weight from 2.7 kDa to 40

kDa(2.7,4.2,6.5,10,15,20,25,30,40kDa),amongthem,40 kDa is orange band, 10 kDa is green band. The labeled apparent molecular weight was calibrated by the molecular weight Marker of standard non-prestained proteins. Using this product, the state of protein electrophoresis and the effect of membrane transfer can be dynamically

observed. After SDS-PAGE electrophoresis, the color bands were transferred to PVDF membrane and NC membrane. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents!

After the prestained protein is combined with the dye, in different buffer systems, there is displacement.  this product is only for reference when judging the molecular weight of the target protein.

Components

Shipping and Storage

The product is shipped with dry ice and can be stored at -30℃ ~ -15℃ for two years.For regular use, it can be placed at 4℃, valid for three months. It is recommended to store in aliquots to avoid repeated freezing and thawing!

Stock solution composition

62.5 mM Tris-H₃PO₄(pH 7.5), 2 mM EDTA, 2% (W/V) SDS, 33% (W/V) Glycerol, 5 mM DTT, 0.02% (V/V) proclin300.

Instructions

1. After the product is thawed at room temperature, mix gently to fully dissolve the precipitate. 

2. Then take an appropriate amount of this product into the gel hole.  mini-gel: 3-5 μL; Western blotting: 1.5-2.5 μL; when the thickness of the gel is greater than 1.5 mm, the loading volume can be appropriately increased.

Notes

1. The product needs to be returned to room temperature before use to fully dissolve the precipitate. Incomplete protein denaturation at low temperature may lead to different degrees of dispersion of electrophoresis bands.

2. In western blot experiments, you have to pay attention to small molecule specificity. At present, conventional transfer buffer 2.7 kDa needs to reduce the current and shorten the time, otherwise it will pass through the membrane.

3. This product contains SDS, and the protein has been denatured, so it should not be used as a molecular weight reference standard for natural protein molecular electrophoresis.

4. The product will have deviations in protein size under different electrophoresis conditions, but after they are calibrated by non-prestained protein standards in the same buffer system, they can be used for protein determination of similar molecular weights.

5. At low concentration of gel, low molecular weight protein will swim on the dye front.

6. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents!

7. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety！

8. For research use only!

 Manuals