Description
BsaI is a Type IIs restriction endonuclease that recognizes non-palindromic sequences and cleaves outside of the recognition site. It is commonly used in Golden Gate assembly, digestion of plasmids to prepare linearized DNA fragments with poly(A/T/G/C) ends, and obtaining specific sticky ends.
Applications
Molecular Cloning
Plasmid Linearization
Specifications
|
Recognition site |
5'-GGTCTC(N)1↓-3' 3'-CCAGAG(N)5↑-5' |
|
Concentration |
20 U/µL |
|
Inactivation Conditions |
The amount of enzyme required to completely digest 1 µg of DNA within 1 hour at 37°C is defined as one unit of activity |
|
Reaction conditions |
React at 37℃ for 1 h |
|
Inactivation conditions |
React at 80℃ for 20 min |
Components
|
Component No. |
Name |
15204ES76 |
15204ES80 |
15204ES90 |
|
15204-A |
BsaI(20 U/μL) |
25 μL |
50 μL |
250 μL |
|
15204-B |
10× BsaI Buffer |
250 μL |
500 μL |
2.5 mL |
Shipping and Storage
The products should be stored at -25℃ ~ -15℃ for 2 years.
Documents:
Safety Data Sheet
Manuals
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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