T4 RNA Ligase 2 (dsRNA Ligase, 10 U/μL) _ 14652ES
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T4 RNA Ligase 2 (dsRNA Ligase, 10 U/μL) _ 14652ES
T4 RNA Ligase 2 (dsRNA Ligase, 10 U/μL) _ 14652ES
T4 RNA Ligase 2, also known as T4 RNA ligase 2, is an ATP-dependent double-stranded RNA ligase that exhibits both inter- and intra molecular ligation activities on RNA strands. Compared to T4 RNA Ligase 1, this enzyme displays a significantly higher activity in ligating nicks in double-stranded RNA than in ligating single-stranded RNA ends. The proximity of a 5'-phosphate group and a 3'-hydroxyl group is required for ligation. Moreover, it has the unique capability to ligate between the 3´-hydroxyl group of RNA and the 5´-phosphate group of DNA within a...

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T4 RNA Ligase 2 (dsRNA Ligase, 10 U/μL) _ 14652ES

YeasenSKU: 14652ES80
Size: 1000 U
Price:
$65.00
Stock:
In stock

T4 RNA Ligase 2, also known as T4 RNA ligase 2, is an ATP-dependent double-stranded RNA ligase that exhibits both inter- and intra molecular ligation activities on RNA strands. Compared to T4 RNA Ligase 1, this enzyme displays a significantly higher activity in ligating nicks in double-stranded RNA than in ligating single-stranded RNA ends. The proximity of a 5'-phosphate group and a 3'-hydroxyl group is required for ligation. Moreover, it has the unique capability to ligate between the 3´-hydroxyl group of RNA and the 5´-phosphate group of DNA within a double-stranded structure.

Features

Strong ligation activity: capable of effectively linking dsRNA substrates.

No residual nuclease, nicking enzyme, or RNase.

Applications

Connecting the cleavage site of dsRNA.

Connecting the 3´ hydroxyl group of RNA to the 5´ phosphate group of double-stranded DNA structure.

Circular RNA preparation.

Specifications

Unit Definition

One unit is defined as the amount of enzyme required to ligate 0.4 µg of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction volume of 20 µl in 30 minutes at 37℃

Source

Recombinant protein expressed by E. coli is derived from the Y10A gene of T4 bacteriophage

Purity

≥95%

Reaction conditions

React at 25℃ for 1 hour

Inactivation conditions

React at 80℃ for 5 min

Components

Components No.

Name

14652ES80

14652ES90

14652-A

T4 RNA ligase 2 (10 U/μL)

100 μL

500 μL

14652-B

10×T4 RL2 Reaction Buffer

500 μL

1.5 mL

Figures

Figure 1. Verification of T4 RNA ligase 2 ligation efficiency.

Note: The 20 μL reaction system contains dsRNA ligation substrates at a final concentration of 20 μM.

Storage

This product should be stored at -25 ~ -15 o C for 1 years.

FAQ

Citations

Documents

Applications

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FAQ

The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.

Certain applications may require additional third-party intellectual property rights.

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