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Hieff™ Super Bst DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I and has been genetically modified to remove its 5’-3’ exonuclease activity. This product possesses strong 5’-3’DNA polymerase activity, strand displacement activity, and dUTP tolerance, making it more suitable for contamination prevention in isothermal amplification reactions such as LAMP, CPA, etc. Compared to Bst Plus DNA Polymerase, Hieff™ Super Bst DNA Polymerase has improved the thermal stability of the Bst enzyme, which helps to enhance the amplification performance of the Bst enzyme. This enzyme is suitable for low-template and high-temperature LAMP reaction systems, effectively improving the specificity of detection and reducing the detection time. The high-temperature resistance characteristic is also applicable for the development of heat-resistant Bst, further enhancing the specificity of detection.
Features
Thermophilic DNA polymerase with strong strand displacement activity
Applications
LAMP, CPA, RCA etc.
High Temperature LAMP/RT-LAMP, Over 70℃
Specifications
|
Polymerase |
Bst DNA Polymerase |
|
Activity Definition |
1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C. |
|
Solution Components |
10 mmol/L Tris-HCl, 50 mmol/L KCl, 0.1 mmol/L EDTA, 1 mmol/L DTT, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25℃. |
Components
|
Name |
14410ES10 20 KU |
14410ES50 100 KU |
14410ES60 200 KU |
|
|
14410 |
Hieff ™ Super Bst DNA Polymerase(2,000 U/μL) |
10 μL |
50 μL |
100 μL |
Figures
Figure 1. RT-LAMP testing with Hieff Super BST at 70℃.
Figure 2. Perfomance of the mutants of Bst DNA polymerase in LAMP asays.
(a) Perormance of the mutants of Bst DNA polymerase in LAMP assays under diferent temperatures. (b) Compared with Bst DNA polymerase of NEB (green), mutants M2 (orange) and M3 (red) exhbited greatly increased activity under different temperatures for diferent targets. (c) Performance of M2 in the fale positive experiment. (d) Performance of M3 in the false positive experiment. (e) Perfomance of the mutants of BST in the thermal acceleration experiment at 50 ℃ for 63 days.
Storage
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Citations
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