Description
Hieff Unicon™ Hotstart J-Taq DNA Polymerase, Glycerol-free (40 U/μL) is a hot-start DNA polymerase. This product utilizes antibody-mediated inhibition, providing high amplification sensitivity and specificity. The inhibitory antibody is completely inactivated and the DNA polymerase activity is fully released after a 30-second heat activation step at the initial denaturation temperature. Using this hot-start Taq enzyme effectively suppresses non-specific amplification caused by primer mis-annealing. This glycerol-free formulation is specifically designed for lyophilized reagent preparation.
Features
Rapid Activation: Taq polymerase achieves full activity after a quick 30 s incubation at 95 °C, ensuring immediate and efficient amplification.
High Sensitivity & Specificity: Provides strong amplification performance even with low template input, minimizing non-specific products.
Broad Instrument Compatibility: Works seamlessly with a wide range of standard PCR and real-time PCR platforms.
Lyophilization-Ready: Formulation supports freeze-drying, enabling long-term storage, reduced shipping costs, and simplified reagent handling.
Components
|
Name |
14326ES02 |
14326ES20 |
14326ES30 |
|
Hotstart J-Taq DNA Polymerase, Glycerol-free ( 40 U/μL) |
50 μL |
500 μL |
2×1 mL |
Storage
This product should be stored at -25~-15℃ for 1 year.
Application
Gene Expression Analysis; Low-Copy Target Amplification; Lyophilized PCR/qPCR.
Figures
1. Consistent Performance & Strong Stability
Figure 1. Stability of Yeasen glycerol-free enzyme (14326ES).
The glycerol-free enzyme showed the same amplification performance as the glycerol-containing enzyme across COVID-19 pseudovirus templates (105–103 copies/mL). After 54 days at 37 °C, its performance remained unchanged, confirming excellent thermal stability.
Documents:
Safety Data Sheet
Manuals
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.