Hifair™ Lyo Multiplex One Step RT-qPCR Kit -11831ES

SKU: 11831ES60

Size: 100 T
Price:
Sale price$155.00

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Description

HifairTM Lyo Multiplex One Step RT-qPCR Kit is a multiplex quantitative PCR kit based on RNA as template. In the process of the experiment, reverse transcription and quantitative PCR were carried out in the same tube, which simplified the experimental operation and reduced the risk of contamination.The unique Buffer and enzyme design ensures that it can be used in the preparation and product design of one-step RT-qPCR lyophilized reaction systems.

In this kit, the first strand cDNA was efficiently synthesized by heat-resistant HifairTM V Reverse Transcriptase and quantitatively amplified by UniconTM HotStart Taq DNA Polymerase. The kit mainly contains optimized lyophilizable buffer, enzymes mix, etc. In addition, the factors that can effectively inhibit the non-specific PCR amplification and improve the amplification efficiency of multiple qPCR reactions are added, which can ensure the amplification efficiency and carry out up to multiple amplification reaction.

This kit is fitted with lyoprotectant that can be used for lyophilization.

Specifications

Hot Start

Built-in hot start

Detection method

Primer-probe detection

PCR method

One step RT-qPCR

Type of sample

RNA

Application equipment

Equipment with RoxABI 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™, StepOne Plus™

Equipment with Low RoxABI 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex

Stratagene MX3000P™, MX3005P™, MX4000P™

Equipment without Rox:

Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™5, MyiQ™, MiniOpticon™, Opticon®, Opticon® 2, Chromo4™

Eppendorf Mastercycler® ep realplex, realplex 2 s; Qiagen Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000

Roche Applied Science LightCycler® 480, LightCycler® 2.0, Lightcycler® 96

Thermo Scientific PikoReal Cycler; Cepheid SmartCycler®Illumina Eco qPCR

Components

Components No.

Name

11831ES60

(100T)

11831ES80

(1,000T)

11831ES92

(10,000T)

11831-A

4×HifairTM V Lyo-Buffer

600 μL

6 mL

60 mL

11831-B

HifairTM Lyo-Enzyme Mix

100 μL

1 mL

10 mL

11831-C

Lyoprotectant

800 μL

8 mL

80 mL

Storage

Part 1:11831-A【4×Hifair V Lyo-Buffer】should be stored at -25℃ ~ -15℃ for 1 year.

Part 2:11831-B【HifairTM Lyo-Enzyme Mix】 should be stored at 2℃ ~ 8℃ for 6 months.

11831-C【Lyoprotectant】should be stored at  -25℃ ~ -15℃ for 1 year.

Figure

Beads performance

Mutiplex PCR

Beads product inquiry

Instructions 

  1. Reaction Composition

Components

Volume (μL)

Final Concentration

4×HifairTM V Lyo-Buffer

6

HifairTM Lyo-Enzyme Mix

1

-

Lyoprotectant

8

-

Primer Mix (10 μM)

1

0.1-1 μM

Probe Mix (10 μM)

0.5

0.05-0.5 μM

Template RNA

5

-

DEPC H2O

to 25

-

Note:Be sure to mix well before use, avoid excessive bubbles caused by violent vibration.

  1. Optimized Cycling Protocol

 

Reaction stage

Temperature

Time

Cycle

1

Reverse transcription

50°Ca

10 min

1

2

Initial denaturation

95°C

2 sec

1

3

Amplification reaction

95°C

1 sec

45 cycles

60°Cb

13 secc

Notes

a) Reverse transcription: The temperature can select 42°C or 50°C for 10-15 minutes.

b) Amplification reaction: The temperature is adjusted according to the Tm value of the designed primers.

c) Fluorescence signal acquisition: Please set the experimental procedure according to the requirements of the instrument manual.

 


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