Description
dTTP, namely 2'-Deoxythymidine-5'-triphosphate, is used for molecular biological reactions, such as PCR, real-time PCR, RT-PCR, cDNA Synthesis, primer extension reaction, DNA sequencing, DNA labeling etc.
This product was in the form of sodium solution, prepared with ultrapure water, adjust pH 7.0 with a high purity NaOH solution. The concentration of dTTP is 100 mmol/L.
Ultra-high purity dTTP: >99% trisphosphate by HPLC; Free of DNase and RNase contamination.
Features
- Greater than 99% purity confirmed by HPLC
- Free of human and E.coli DNA
- Highly stable
Applications
- Long-range PCR (20 kb)
- cDNA synthesis and RT-PCR
- Real-time PCR
- Standard PCR
- High fidelity PCR
Specifications
| Concentration | 100 mM |
| CAS NO. | 18423-43-3 |
| Form | Liquid |
| Purity | >99% |
| Label or Dye | Unlabeled |
| For Use With (Application) | PCR Amplification |
Components
| Components No. | Name | 10120ES74 | 10120ES80 | 10120ES96 | 10120ES97 |
| 10120 | dTTP Solution (100 mmol/L) | 400 μL | 1 mL | 25 mL | 400 mL |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 2 years. Please avoid repeated freeze-thaw.
Documents:
Citations & References:
[1] Zhang J, Zhang G, Zhang W, et al. Loss of RBMS1 promotes anti-tumor immunity through enabling PD-L1 checkpoint blockade in triple-negative breast cancer [published online ahead of print, 2022 May 10]. Cell Death Differ. 2022;10.1038/s41418-022-01012-0. doi:10.1038/s41418-022-01012-0(IF:15.828)
[2] Chai Q, Wang X, Qiang L, et al. A Mycobacterium tuberculosis surface protein recruits ubiquitin to trigger host xenophagy. Nat Commun. 2019;10(1):1973. Published 2019 Apr 29. doi:10.1038/s41467-019-09955-8(IF:11.878)
[3] Wang X, Ni J, You Y, et al. SNX10-mediated LPS sensing causes intestinal barrier dysfunction via a caspase-5-dependent signaling cascade. EMBO J. 2021;40(24):e108080. doi:10.15252/embj.2021108080(IF:11.598)
[4] Feng Y, Wang Y, Wang X, et al. Simultaneous epigenetic perturbation and genome imaging reveal distinct roles of H3K9me3 in chromatin architecture and transcription. Genome Biol. 2020;21(1):296. Published 2020 Dec 8. doi:10.1186/s13059-020-02201-1(IF:10.806)
[5] Tao X, Wan X, Wu D, Song E, Song Y. A tandem activation of NLRP3 inflammasome induced by copper oxide nanoparticles and dissolved copper ion in J774A.1 macrophage. J Hazard Mater. 2021;411:125134. doi:10.1016/j.jhazmat.2021.125134(IF:10.588)
[6] Alolga RN, Opoku-Damoah Y, Alagpulinsa DA, et al. Metabolomic and transcriptomic analyses of the anti-rheumatoid arthritis potential of xylopic acid in a bioinspired lipoprotein nanoformulation. Biomaterials. 2021;268:120482. doi:10.1016/j.biomaterials.2020.120482(IF:10.317)
[7] Mo X, Du S, Chen X, et al. Lactate Induces Production of the tRNA<sup>His</sup> Half to Promote B-lymphoblastic Cell Proliferation. Mol Ther. 2020;28(11):2442-2457. doi:10.1016/j.ymthe.2020.09.010(IF:8.986)
[8] Hao W, Han J, Chu Y, et al. Lower fluidity of supported lipid bilayers promotes neuronal differentiation of neural stem cells by enhancing focal adhesion formation. Biomaterials. 2018;161:106-116. doi:10.1016/j.biomaterials.2018.01.034(IF:8.806)
[9] Lin Z, Xia S, Liang Y, et al. LXR activation potentiates sorafenib sensitivity in HCC by activating microRNA-378a transcription. Theranostics. 2020;10(19):8834-8850. Published 2020 Jul 11. doi:10.7150/thno.45158(IF:8.579)
[10] Liu H, Xing R, Ou Z, et al. G-protein-coupled receptor GPR17 inhibits glioma development by increasing polycomb repressive complex 1-mediated ROS production. Cell Death Dis. 2021;12(6):610. Published 2021 Jun 12. doi:10.1038/s41419-021-03897-0(IF:8.469)
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
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