Effortless DNA extraction from Gram-negative bacteria, Gram-positive bacteria, and fungi.

The MolPure™ Magnetic Bacterial DNA Kit provides a simple and rapid solution for extracting genomic DNA from Gram-negative bacteria, Gram-positive bacteria, and fungi. The kit employs super-paramagnetic bead-based purification technology and requires no toxic phenol/chloroform extraction. The workflow is straightforward, and the purified DNA is directly compatible with restriction digestion, PCR, sequencing, and labeling applications.

Features

• High yield and excellent purity: specific lysis beads provide more complete cell-wall disruption, yielding DNA of high purity and high recovery.

• High efficiency: lysozyme incubation is not required, and high-quality DNA can be obtained within a short hands-on time, enabling seamless integration with liquid-handling workstations or fully automated nucleic-acid extractors for rapid, large-scale processing.

• Excellent safety: the workflow avoids toxic organic solvents including phenol, chloroform, and β-mercaptoethanol.

Applications

 Figure 1. DNA extraction from eight 7–8 ml bacterial cultures (centrifuged cell pellets), processed with 350 μl lysis buffer, 20 μl Proteinase K, and 10 μl RNase A, homogenized on a Retsch MM400 at 30 Hz for 5 min, incubated at 65°C for 10 min, and purified on a 48-channel automated extractor (70 μl elution). Gram-negative species (E. coli, Salmonella, Shigella) yielded Qubit concentrations >120 ng/μl; Gram-positive species (S. aureus, L. monocytogenes) were also successfully detected.

Figure 2. DNA extraction from six yeast cultures (350 μl lysis buffer, 20 μl Proteinase K, 10 μl RNase A; Retsch MM400 at 30 Hz for 5 min; 65°C for 10 min; 70 μL elution). The kit (18565ES) yields high concentrations with excellent purity, no RNA carry-over, and bright, sharp electrophoresis bands. Nanodrop A260/A280 ratios: 1.79–1.95; A260/A230 ratios: 1.71–1.84.

Storage

Part I: shipped at room temperature and stored at 2 to 8°C; shelf life 18 months.

Part II: shipped at room temperature and stored at room temperature protected from light; shelf life 18 months.