T7 High Yield RNA Synthesis Kit (low dsRNA) optimizes the transcription reaction system. The T7 RNA polymerase used in this kit is an engineered and optimized variant of wild-type T7 RNA polymerase, obtained by mutation, which can significantly reduce dsRNA content. Using linear double-stranded DNA containing a T7 promoter sequence as template and NTPs as substrates, it transcribes the DNA sequence downstream of the promoter and efficiently synthesizes single-stranded RNA. Modified nucleotides can be incorporated into the substrates during transcription to prepare biotin- or dye-labeled RNA.
This kit can synthesize both long and short transcripts. With 1 μg of template input, 100–200 μg of RNA can be produced. The synthesized RNA can be used for a variety of downstream applications such as RNA structure and function studies, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation.

Product Information

Component Information

Feature

Extremely high yield—up to 200 μg in 2 hours

Broad applicability—suitable for transcription of 20 nt to 10,000 nt RNA

Extremely low dsRNA—significantly reduces dsRNA content during IVT

Multiple applications—suitable for standard, labeled, and modified RNA synthesis

Applications

1. Under different length applications, both 2K and 4K fragments achieved yields of ~11 mg/mL; with the low dsRNA T7 system, dsRNA content was drastically reduced.

2. High integrity

Figure 1: 44–4000 nt RNA analyzed on the Agilent 2100 Bioanalyzer. The electrophoretic bands are single and the peaks are distinct and sharp, indicating high RNA integrity.