DNA Library Preparation Solutions
Advancing DNA Library Accuracy by Enzyme Engineering
Compatiable with illumina, MGI and Element sequencing platform
Key Performance Data
Enhanced library conversion, enabling high-quality sequencing data.
(A). Enhanced library conversion efficiency with Yeasen DNA Library Prep Kit, delivering higher library yield from low-input DNA.
(B). Ultra-low adapter dimer formation, demonstrating minimal self-ligation even with as little as 0.5 ng gDNA input.
Compared with multiple commercial ligases, Yeasen’s ligation chemistry showed minimal adapter self-ligation and the lowest adapter dimer formation at 0.5 ng gDNA input, enabling high-quality libraries from ultra-low DNA amounts.
Low fragmentation and GC bias ensure uniform coverage
(A). Cleavage-site preference analysis.
Base-quality window analysis (30 bp, Read 1 and Read 2) showed that Yeasen random endonuclease–based fragmentation has lower sequence bias than the Tn5 transposase method and produces profiles closer to ultrasonic fragmentation.
(B). Reduced sequence artifacts enables high-sensitivity applications.
Library preparation using 200 ng NA12878 gDNA showed that the Yeasen 12194ES kit produced a lower percentage of adapter self-ligation and mismatched reads compared with supplier K* and V*.
Reduced ligation- and fragmentation-associated artifacts improve data accuracy and enhance confidence in sensitive applications, including low-allele-frequency variant detection.
Reduced artifacts improved data quality and supported more accurate SNP and InDel detection, reflected by slightly higher F-measure scores compared with competing kits.
(C). GC bias analysis using 1 μg NA12878 DNA (30 °C, 10 min enzymatic fragmentation; PCR-free library; PE150 sequencing, 30×, 90 Gb data) showed consistent coverage across both low-GC and high-GC regions, demonstrating uniform performance across genomes with GC contents ranging from 30% to 75%.
Application
Yeasen provides application-driven NGS workflows designed to support diverse research and clinical needs—from fundamental genomics to translational and applied sequencing studies.
Scientific Research Services
NGS solutions for basic and translational research across genomics and transcriptomics.
Agricultural Genomics & Breeding
Sequencing solutions for crop and animal genomics, breeding, and marker-assisted selection.
Pathogen & Microbial Sequencing
NGS workflows for pathogen detection, microbiome, and metagenomic analysis.
Reproductive & Genetic Disease Research
Sequencing solutions for inherited disease research and reproductive genetics.
Tumor Detection & Targeted Gene Detection
Targeted sequencing solutions for tumor profiling and gene mutation analysis.
Methylation-Based Solutions for Early Cancer Screenin
NGS solutions for DNA methylation analysis and early cancer detection.
Biomedical Applications
NGS solutions for Biologics Safety and Quality Control
Product Selection Guide
Application | Cat.No. | Type | Input | Supported Samples | Low-Frequency Variant Detection | PCR-Free Compatible | Workflow Time |
Universal | Mechanical | 100 pg –1 μg | | ★★★★★ | ✔ | ~2.5–2.75 h | |
Universal | Enzymatic | 1 ng– 1 μg | cfDNA / gDNA / FFPE | ★★ | ✔ | ~2.25–2.5 h | |
Genetic | Enzymatic | 1 ng–1 μg | Blood / FFPE gDNA | ★★★★ | ✔ | ~2.25–2.5 h | |
Pathogen | Enzymatic | 100 pg– 500 ng | Microbial/Environmental gDNA/cDNA | ★★ | ✔ | ~1.75 h | |
Methylation Analysis | Mechanical | 10 ng– 1μg | cfDNA / gDNA/ FFPE | ★ | ✔ | ~2.25 h |
Customer Cases & Protocols
FAQs
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